Nod Scid Mice
Nod Scid Mice - Related Videos
07:10
Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies
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Cited by 13 •
2019
This protocol provides a method to establish humanized mice (hu-NSG) via intrahepatic injection of human hematopoietic stem cells into radiation-conditioned neonatal NSG mice. The hu-NSG mouse is susceptible to HIV infection and combinatorial antiretroviral therapy (cART) and serves as a suitable pathophysiological model for HIV replication and latency investigations.
11:24
Intrasplenic Transplantation of Hepatocytes After Partial Hepatectomy in NOD.SCID Mice
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Cited by 1 •
2018
We have described a protocol for performing partial hepatectomy (PHx) and cell transplantation via spleen in NOD.SCID (NOD.CB17-Prkdcscid/J) mice. In this protocol, an incision is made to expose and resect the left lobe of the liver followed by another incision for the intrasplenic transplantation of cells.
10:43
Transplantation of Pancreatic Islets Into the Kidney Capsule of Diabetic Mice
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Cited by 98 •
2007
Our protocol was developed to cleanly and easily deliver islets or cells under the kidney capsule of mice. Cells are concentrated into pellets in the final tubing used for transplanting the cells under the kidney capsule. The ease of this technique reduces stress to the cells and the mouse.
04:16
Imaging Transgenic Beta-Cells in an Immunodeficient Mouse Challenged with Diabetic Splenocytes
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2025
This video demonstrates an imaging-based method to visualize transplanted luciferase-expressing beta cells into a non-obese diabetic immunodeficient mouse. The introduction of splenocytes from a diabetic mouse leads to progressive beta cell destruction, visually confirmed by declining luminescence after substrate introduction.
02:26
A Technique to Generate a Human PBMC-Engrafted Humanized Xenograft Mouse Model
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2025
This video demonstrates an assay to generate a humanized xenograft mouse model. An immunocompromised mouse is injected with cyclophosphamide to cause immune cell depletion. Patient-derived tumor cells mixed with human peripheral blood mononuclear cells (PBMCs) in a hydrogel are subcutaneously injected into the mouse, forming a tumor with infiltrating T cells.
FAQs
The scid mutation disrupts V(D)J recombination, the genetic rearrangement process required to generate diverse antigen receptors. Without effective V(D)J recombination, mature T and B lymphocytes do not develop. This defect provides a defined model for examining immune function and for testing whether transplanted cells can persist without rejection by these adaptive immune populations.
The NOD background adds defects in innate immune responses that are distinct from the scid-associated loss of mature T and B lymphocytes. In particular, NOD scid mice show reduced natural killer-cell activity and impaired complement function. These combined abnormalities help explain their limited immune rejection and support experiments involving transplanted human or mouse cells.
Human-cell xenografts can be maintained because NOD scid mice combine absent mature T and B lymphocytes with reduced natural killer-cell activity and complement function. Together, these immune limitations reduce rejection of transplanted material. As a result, researchers can study human cell behavior in vivo rather than examining the cells only in isolated culture systems.
Failure of V(D)J recombination removes a central source of antigen-receptor diversity and prevents mature T- and B-cell development in this model. That connection makes NOD scid mice useful for linking a defined genetic defect to immune-system function. It also creates an experimental setting in which transplanted cells can be evaluated with limited adaptive immune interference.
Researchers use these mice to examine transplanted human or mouse cells in vivo, including xenografts and tumor models. The same engraftment capacity supports infectious-disease studies, stem-cell research, and investigations of human cell behavior. Their usefulness therefore extends beyond one disease area, allowing diverse biological processes and experimental cell populations to be studied in a living host.
Their ability to accept many transplanted human cells provides a foundation for developing humanized immune-system models. In this context, engrafted human cells can be studied within a living mouse rather than separately from host biology. Such models connect immune-function research with studies of human cell behavior and can also support preclinical evaluation of therapies.
NOD scid mice can support preclinical evaluation of therapies by allowing researchers to observe responses in an in vivo setting containing transplanted human or mouse cells. They are also relevant to tumor biology, infectious-disease research, and stem-cell studies. The resulting experiments can connect treatment or biological effects with cell behavior and engraftment in the host.