Pit And Fissure Sealing
Pit And Fissure Sealing - Related Videos
04:41
Optimized Sealing Process and Real-Time Monitoring of Glass-to-Metal Seal Structures
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Cited by 7 •
2019
Key procedures to optimize the sealing process and achieve real-time monitoring of the metal-to-glass seal (MTGS) structure are described in detail. The embedded fiber Bragg grating (FBG) sensor is designed to achieve online monitoring of temperature and high-level residual stress in the MTGS with simultaneous environmental pressure monitoring.
04:46
Resorption Pit Assay: An In Vitro Technique to Quantify Calcium Resorption Activity of Mature Osteoclasts using Calcium Phosphate-Coated Culture Plates
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2025
In this video, we describe an in vitro osteoclastic resorption assay on calcium phosphate-coated cell culture plates cultured with human peripheral blood mononuclear cell-derived osteoclasts. The osteoclasts form a characteristic actin ring on the calcium phosphate-coated matrix of the cell culture plate and degrade the matrix within the actin ring region to release calcium and phosphate ions, resulting in the formation of distinct resorption pits within the degraded matrix.
07:51
The C-seal: A Biofragmentable Drain Protecting the Stapled Colorectal Anastomosis from Leakage
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Cited by 8 •
2010
The C-seal is a biofragmentable drain protecting the stapled colorectal anastomosis. In this video, details of the manufacturing, deployment and use are shown.
06:10
Accessible Silicone Chip-to-Membrane Sealing Procedure for Flexible, Reliable Bonding
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2026
A novel sealing protocol to produce water-tight silicone-based seals for membranes of Polydimethylsiloxane (PDMS) chip devices. Intended for labs setting-up chip models and small-scale chip platform development. We demonstrate the protocol using plastic and native tissue membranes. This procedure only requires PDMS, toluene, mold, membrane, and a vacuum chamber.
07:03
A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro
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Cited by 2 •
2022
Here, we present a simple and effective assay procedure for resorption pit assays using calcium phosphate coated cell culture plates.
FAQs
Pit and fissure sealing interrupts the sequence linking plaque retention, bacterial activity, acid production, and enamel demineralization. By covering irregular occlusal surfaces, the sealant changes the local environment where bacteria and fermentable food substances would otherwise remain in contact with enamel. This mechanism helps reduce the conditions that initiate occlusal caries.
Narrow pits and fissures can provide sheltered surface irregularities where plaque accumulates and remains close to enamel. Their shape therefore creates a local setting favorable to the microbial processes associated with decay. Covering these grooves reduces bacterial retention at the site most vulnerable to plaque-associated acid effects, particularly on molar chewing surfaces.
Both material types serve as physical coverings for tooth-surface irregularities, but they reach that state differently: resin-based material hardens, whereas glass ionomer material sets. Once established over the pits and fissures, either material forms a barrier that limits bacterial retention and contact between fermentable food substances and enamel.
Changing the local surface environment matters because caries development depends on interactions among retained plaque, bacterial activity, fermentable substances, and enamel. A sealant does not need to remove every biological factor to be useful. By interrupting their contact at vulnerable grooves, it reduces the local conditions that support acid-related enamel damage.
The procedure places a resin-based or glass ionomer material into the tooth's narrow pits and fissures. The material flows into these irregularities, then hardens or sets to create a continuous covering over the vulnerable surface. Its immediate functional outcome is a barrier that reduces plaque-associated retention and contact with enamel.
Sealants are particularly relevant for newly erupted permanent molars with deep fissures. These teeth contain occlusal grooves where plaque can accumulate and initiate decay, making surface protection biologically meaningful. Applying the barrier to such irregularities supports prevention by reducing the local opportunities for bacterial retention, acid production, and enamel demineralization.
The expected preventive outcome is a lower likelihood that plaque-associated microbial activity will produce the conditions needed for occlusal caries. By limiting retention and contact at deep grooves, sealing helps protect enamel from acid-related demineralization. In practical oral-health research, this links a localized surface intervention with preservation of tooth structure.
The procedure illustrates how modifying a physical microenvironment can interrupt a biological disease process. Rather than targeting the entire oral microbial community, it changes a specific tooth surface where plaque, food substrates, bacterial activity, and enamel interact. This localized approach demonstrates how surface structure can influence microbial disease development and tissue preservation.