Real-time Blood Flow Quantification
Real-time Blood Flow Quantification - Related Videos
02:58
Laser Doppler Needle Probe Insertion: A Technique for Real-time Quantification of Blood Flow within the Spinal Cord in a Porcine Model
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2025
This video demonstrates the placement of a laser Doppler needle probe in the spinal cord of a porcine model. This helps in the continuous quantification of blood supply to the spinal cord, an important assessment in spinal cord injury.
06:16
Real-time Imaging and Quantification of Fungal Biofilm Development Using a Two-Phase Recirculating Flow System
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2018
We describe the assembly, operation, and cleaning of a flow apparatus designed to image fungal biofilm formation in real time while under flow. We also provide and discuss quantitative algorithms to be used on the acquired images.
03:38
Developing a Cerebral Blood Flow Atlas for Blood Flow Mapping
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2025
Source: Tan, Z., et al., Construction and Application of Cerebral Functional Region-Based Cerebral Blood Flow Atlas Using Magnetic Resonance Imaging-Arterial Spin Labeling. J. Vis. Exp. (2024).This video demonstrates the method to construct a cerebral blood flow atlas by integrating magnetic resonance imaging or MRI-arterial spin labeling data with segmented brain regions, visualizing blood flow patterns, and quantifying flow levels across functional areas.
09:38
A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time
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Cited by 13 •
2017
This paper describes an experimental model of hemostasis that simultaneously measures platelet function and coagulation. Platelet and fibrin fluorescence is measured in real-time, and platelet adhesion rate, coagulation rate, and onset of coagulation are determined. The model is used to determine platelet procoagulant properties under flow in concentrates for transfusion.
11:59
In Vivo Real-Time Study of Drug Effects on Carotid Blood Flow in the Ovine Fetus
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2023
The present protocol describes a method to deliver drugs and gene expression-modifying agents perivascularly in an in utero developing fetus. Importantly, the effect of drugs/agents on blood flow can be measured with the progression of pregnancy.
FAQs
In Doppler ultrasound, moving red blood cells scatter emitted sound waves, producing a frequency shift that can be analyzed to estimate blood velocity. The measurement therefore tracks circulation as it changes rather than treating flow as a fixed property. This makes it useful for observing dynamic cardiovascular or tissue responses in living systems.
A velocity reading describes how quickly blood moves, but it does not by itself establish how much blood passes through a vessel. Vessel diameter or other imaging data can be combined with velocity to support volumetric flow calculations. This distinction helps interpret whether an apparent change reflects movement speed, transported volume, or both.
These related approaches extend measurement beyond Doppler ultrasound by assessing microvascular perfusion or regional circulation. Their value is contextual: a study focused on small-vessel tissue perfusion may require information different from a velocity estimate in a vessel. Comparing the signals can help align the measurement method with the biological scale and circulation pattern under investigation.
Researchers monitor circulation with a suitable signal, then analyze resulting changes in velocity, volume, or perfusion over time. When volumetric flow is needed, vessel diameter or imaging information supports the calculation. The time-dependent pattern can then be examined in relation to a drug treatment or physiological challenge, revealing how circulation responds while the condition changes.
These measurements can reveal how vascular regulation changes circulation and how effectively tissues receive oxygen. They also support investigation of disease-related vascular changes and the effects of drugs or physiological challenges. Because the measurements are dynamic, researchers can examine not only a final value but also how cardiovascular or regional tissue function changes during the condition being studied.
Interpretation requires identifying which quantity changed: velocity, volumetric flow, or perfusion. A velocity shift alone does not specify transported blood volume unless vessel diameter or imaging data are considered, while perfusion measures can describe microvascular or regional circulation. Keeping these outputs distinct prevents researchers from treating different indicators of circulation as interchangeable.