Human Recombinant Vitronectin
Human Recombinant Vitronectin - Related Videos
02:55
Determining the Role of Vitronectin in Bacterial Resistance to Bactericidal Activity of Human Serum
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2025
This video demonstrates the role of vitronectin, a human serum glycoprotein, on bacterial resistance. Antibodies in the serum interact with bacterial surface proteins, activating complement proteins and forming the membrane attack complex, causing bacterial cell lysis. Vitronectin binding on bacterial membrane protein prevents the membrane attack complex formation, resulting in bacterial survival.
03:05
Detection of Vitronectin Binding to Bacterial Surfaces using Flow Cytometry
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2025
This video demonstrates the detection of vitronectin's interaction with bacterial surface proteins. The flow-cytometry analysis measures the fluorescence signal of fluorescently labeled antibodies bound to vitronectin, confirming its presence at the bacterial surface. This technique provides insights into host-pathogen interactions and potential bacterial infection therapies.
03:53
An Assay to Study the Role of Vitronectin in Bacterial Adherence to Host Epithelial Cells
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2025
In this video, we demonstrate an assay to study the role of vitronectin in Haemophilus influenzae adherence to the host epithelial cells.
02:01
Purification of Bacteria-Derived Recombinant P Domain Proteins of Human Norovirus
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2025
Source: Leuthold, M. M., et al. Production of Human Norovirus Protruding Domains in E. coli for X-ray Crystallography. J. Vis. Exp. (2016).This video demonstrates the purification of recombinant human norovirus P domain using size exclusion chromatography, highlighting the separation of the target protein from higher and lower molecular weight impurities based on differential pore accessibility. The process is monitored by UV absorbance and confirmed through SDS-PAGE analysis of eluted...
14:23
Recombineering Homologous Recombination Constructs in Drosophila
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Cited by 9 •
2013
Homologous recombination techniques greatly advance Drosophila genetics by enabling the creation of molecularly precise mutations. The recent adoption of recombineering allows one to manipulate large pieces of DNA and transform them into Drosophila6. The methods presented here combine these techniques to rapidly generate large homologous recombination vectors.
FAQs
The RGD sequence provides a recognition site for cell-surface integrins. When integrins engage this sequence, they connect extracellular attachment with intracellular signaling pathways. Those signals can affect whether cells remain viable, proliferate, migrate, or adopt differentiated states. In developmental biology, this molecular connection helps explain how a defined culture surface can influence stem-cell behavior without changing the cells’ genetic program directly.
A defined surface supplies a consistent extracellular matrix cue across culture conditions. That consistency reduces variation arising from poorly characterized or animal-derived matrix preparations, making differences in cell growth or differentiation easier to attribute to the experimental treatment. For developmental studies, controlling this environmental variable strengthens comparisons between experiments and supports more reproducible analysis of developmental transitions.
Human recombinant vitronectin supports a xeno-free culture environment, whereas animal-derived matrices can introduce biological variability associated with their source and composition. Using the recombinant protein therefore gives researchers a more controlled matrix component while retaining integrin-mediated attachment and signaling. This distinction matters when developmental experiments require reproducible conditions or when investigators want to reduce contributions from nonhuman materials.
It provides a defined attachment environment that can support the maintenance of human pluripotent stem cells and their directed differentiation into specialized cell types. The same matrix cue can therefore be used while cells are expanded and as researchers guide developmental changes. Its value lies in connecting routine culture support with a more controlled system for studying cell-fate decisions.
Researchers can incorporate Human Recombinant Vitronectin into systems that model early human development and generate specialized cell types from pluripotent stem cells. The controlled extracellular environment helps investigators examine how cells respond during maintenance and directed differentiation. These applications make the material relevant both to developmental modeling and to studies seeking reproducible stem-cell-based experimental outcomes.
Studies can assess changes in cell attachment, survival, proliferation, migration, and differentiation under defined culture conditions. In stem-cell experiments, these readouts help determine whether cells remain suitably maintained or progress toward a desired specialized identity. Because the matrix component is consistent, observed outcomes can be compared more reliably across experiments and used to evaluate developmental models.