Fiber Pulling Technique

Fiber pulling technique is an engineering method for producing a uniform fiber or a tapered fiber by heating a material and drawing it under controlled tension. In optical-fiber fabrication, localized heating softens glass while coordinated motion of the pulling stages stretches the softened region, determining the fiber’s diameter, taper profile, and mechanical properties. The process supports the manufacture of optical waveguides, tapered fiber couplers, microstructured fibers, and fiber-based sensors. Precise control of temperature, pulling speed, and tension helps achieve consistent geometry and optical performance, making fiber pulling important in telecommunications, photonics research, and advanced sensing systems.

Fiber Pulling Technique - Related Videos

Research

JoVE Journal - Biology

Pull-down of Calmodulin-binding Proteins

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Cited by 19 •

2012

Calmodulin (CaM) pull-down assay is an effective way to investigate the interaction of CaM with various proteins. This method uses CaM-sepharose beads for efficient and specific analysis of CaM-binding proteins. This provides an important tool to explore CaM signaling in cellular function.

Education

JoVE Science Education - Chemistry

Co-Immunoprecipitation and Pull-Down Assays

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2023

Co-immunoprecipitation (CoIP) and pull-down assays are closely related methods to identify stable protein-protein interactions. These methods are related to immunoprecipitation, a method for separating a target protein bound to an antibody from unbound proteins. In CoIP, an antibody-bound protein is itself bound to another protein that does not bind with the antibody, this is followed by a separation process that preserves the protein-protein complex. The difference in pull-down assays is that...

Imaging Serotonergic Fibers in the Mouse Spinal Cord Using the CLARITY/CUBIC Technique

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Cited by 13 •

2016

Supraspinal projections are important for pain perception and other behaviors, and serotonergic fibers are one of these fiber systems. The present study focused on the application of the combined CLARITY/CUBIC protocol to the mouse spinal cord in order to investigate the termination of these serotonergic fibers.

Research

JoVE Journal - Biology
Free Sample

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

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Cited by 73 •

2011

DT40, a model vertebrate genetic system, provides a powerful tool to analyze protein function. Here we describe a simple method that allows qualitative analysis of parameters that influence DNA synthesis during the S-phase in DT40 cells at the single molecule level.

Microfluidic Dry-spinning and Characterization of Regenerated Silk Fibroin Fibers

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Cited by 6 •

2017

A protocol for the microfluidic spinning and microstructure characterization of regenerated silk fibroin monofilament is presented.

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