Knife Edge Setup
Knife Edge Setup - Related Videos
10:25
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The full process from brain specimen preparation to serial sectioning imaging using the Knife-Edge Scanning Microscope, to data visualization and analysis is described. This technique is currently used to acquire mouse brain data, but it is applicable to other organs, other species.
09:23
A Low Cost Setup for Behavioral Audiometry in Rodents
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A fast and inexpensive method for the behavioral determination of hearing parameters like hearing thresholds, hearing impairments or phantom perceptions (subjective tinnitus) is described. It uses pre-pulse inhibition of the acoustic startle response and can be easily implemented in a personal computer using a programmable AD/DA-converter and a piezo sensor.
10:13
Hemi-laryngeal Setup for Studying Vocal Fold Vibration in Three Dimensions
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Cited by 12 •
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This paper introduces a protocol for the preparation of hemi-larynx specimens facilitating a multi-dimensional view of vocal fold vibration, in order to investigate various biophysical aspects of voice production in humans and non-human mammals.
05:52
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Cited by 5 •
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The protocol presented here describes the detailed operation method and proves the effectiveness of a small needle knife in the treatment of frozen shoulders.
06:42
Use of the Scissor-Type Knife During the Peroral Endoscopy Myotomy Procedure for the Treatment of Achalasia
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To minimize the technical difficulty and improve the safety of peroral endoscopic myotomy (POEM), we describe a protocol for using a scissor-type knife for the main steps of POEM, including mucosal incision, submucosal tunneling, myotomy, and hemostasis.
FAQs
The detector’s power reading changes as the edge moves through successive portions of the beam. Plotting transmitted power against edge position creates a response curve whose shape reflects how light is distributed across the beam. Interpreting that curve allows engineers to estimate the beam’s spatial intensity profile and evaluate its size without relying on camera imaging.
The blade provides a sharp, well-defined boundary for blocking light, while the opaque edge prevents the blocked portion from reaching the detector. Translating this boundary gradually changes the amount of transmitted power rather than producing only an all-or-nothing measurement. That controlled change gives the recorded curve spatial information about the beam and its behavior in the optical system.
Unlike camera-based profiling, a knife edge setup does not require a camera to capture the beam image. It uses progressive edge translation and detector power readings, making it a simple, low-cost option for engineering measurements. The resulting information comes from analyzing a transmitted-power curve rather than directly viewing and processing a camera image of the beam.
Place the sharp blade or opaque edge in the beam path, translate it progressively across the beam, and record transmitted power at each edge position. The collected power-versus-position data are then analyzed as a curve. This workflow supports evaluation of the beam’s profile and size, as well as focus position and alignment for the optical system under test.
Analysis of the measured power curve provides information about where the beam reaches focus and how the optical arrangement is aligned. Engineers can use these results to assess beam behavior around a focused region and identify whether adjustments are needed. This makes the technique useful for lens and mirror alignment and for optimizing focused laser systems.
Engineering applications include laser beam characterization, lens and mirror alignment, optical-system quality control, and optimization of focused laser systems. Its simple measurement arrangement and low cost make it practical when a camera-based profiler is unnecessary or unavailable. The resulting curve supplies measurable evidence for evaluating beam size, focus position, spatial profile, and alignment.