Multi-scale Feature Extraction
Multi-scale Feature Extraction - Related Videos
08:05
Small-scale Nuclear Extracts for Functional Assays of Gene-expression Machineries
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Cited by 37 •
2012
A protocol for preparation of robust, small-scale HeLa nuclear extracts is described. This protocol is valuable for assays that require use of small populations of cells, such as cells treated with drugs or RNAi. The method should be applicable to a wide variety of gene expression assays and other cell types, including patient cells.
08:46
Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
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Cited by 5 •
2016
For this study synchrotron radiation micro-tomography, a non-destructive three-dimensional imaging technique, is employed to investigate an entire microelectronic package with a cross-sectional area of 16 x 16 mm. Due to the synchrotron's high flux and brightness the sample was imaged in just 3 min with an 8.7 µm spatial resolution.
09:16
Medium-scale Preparation of Drosophila Embryo Extracts for Proteomic Experiments
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Cited by 4 •
2017
The goal of this protocol is to provide a straightforward and inexpensive approach to collecting Drosophila embryos at medium scale (0.5-1 g) and preparing protein extracts that can be used in downstream proteomic applications, such as affinity purification-mass spectrometry (AP-MS).
12:19
Multi-Scale Modification of Metallic Implants With Pore Gradients, Polyelectrolytes and Their Indirect Monitoring In vivo
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Cited by 4 •
2013
In this video, we will demonstrate modification techniques for porous metallic implants to improve their functionality and to control cell migration. Techniques include development of pore gradients to control cell movement in 3D and production of basement membrane mimics to control cell movement in 2-D. Also, a HPLC-based method for monitoring implant integration in-vivo via analysis of blood proteins is described.
10:06
Characterization Of Multi-layered Fish Scales (Atractosteus spatula) Using Nanoindentation, X-ray CT, FTIR, and SEM
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Cited by 15 •
2014
This paper presents the methods used for probing spatially correlated chemical, structural, and mechanical properties of the multilayered scale of Atractosteus spatula (A. spatula) using nanoindentation, Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and X-ray computed tomography (X-ray CT). The experimental results have been used to investigate the design principles of protective biological materials.
FAQs
Each scale-specific operation emphasizes patterns visible at a particular resolution or frequency range. Filters and transforms can produce separate representations, while neural-network layers can build features across successive processing stages. Combining these outputs allows an engineering system to retain localized details without losing broader structural information, which is important when the relevant signal appears at more than one scale.
Local features can describe fine details such as small defects or limited image regions, whereas global features represent larger shapes, arrangements, or spatial structure. Using both types reduces dependence on a single pattern size or frequency. As a result, the representation can remain useful when objects, defects, or physical phenomena vary in scale within the same engineering dataset.
Single-resolution analysis may emphasize either detailed patterns or broader organization, depending on the selected scale. A multi-scale approach retains information from several resolutions and combines the resulting features. This broader representation supports more reliable interpretation when the target structure is unknown in advance or when engineering measurements contain both small-scale details and large-scale patterns.
Useful scales depend on the sizes and frequencies of the objects, defects, or physical phenomena being examined. The selected filters, transforms, or processing layers should therefore produce representations that cover the relevant range of detail and structure. Matching the scale range to the engineering data helps preserve informative patterns for later detection, classification, segmentation, or prediction.
A typical workflow begins with a signal, image, or spatial measurement, followed by processing at several selected resolutions using filters, transforms, or neural-network layers. The resulting scale-specific features are then combined into a representation containing local and global characteristics. That representation can subsequently support tasks such as identifying patterns, separating regions, classifying observations, or making predictions.
The method can be applied to signals, images, and spatial measurements. Its supported engineering uses include image analysis, computer vision, condition monitoring, remote sensing, and pattern recognition. In these settings, multi-scale representations can assist with detecting objects or defects, classifying patterns, segmenting image regions, and predicting outcomes when relevant structures occur at different sizes or frequencies.
Condition-monitoring systems can use the combined representation to capture both localized indications and broader patterns in measured data. This is relevant when defects or physical changes may appear at different sizes or frequencies. The extracted features can then support detection or classification, helping distinguish meaningful engineering patterns from the wider structural context recorded in signals, images, or spatial measurements.