Peripheral Blood Collection
Peripheral Blood Collection - Related Videos
02:51
A Mouse Tail Snip Procedure for Peripheral Blood Collection and Plasma Isolation
0 Views •
2025
In this video, we demonstrate a tail-snipping procedure in a mouse model to collect peripheral blood. The collected blood is subsequently subjected to centrifugation to separate plasma, which can be used for further experiments.
01:30
Blood Collection in Rabbits
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2026
All animal procedures described here must be conducted in accordance with institutional animal ethics guidelines and approved by IACUC. All procedures must follow the principles of the 3Rs—Replacement, Reduction, and Refinement—and must be performed by trained personnel. Blood sampling is commonly performed in laboratory rabbits for research and clinical applications. The collection site is selected based on the required sample volume, sampling frequency, and study objectives. As a general...
05:12
Repeated Blood Collection from Tail Vein of Non-Anesthetized Rats with a Vacuum Blood Collection System
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Cited by 25 •
2017
Here, we describe a simple tail vein blood sampling method in non-anesthetized rats using a vacuum extraction tube system. This method reduces the risk of direct exposure to blood and simplifies taking multiple samples from a single venipuncture.
01:33
Blood Collection in a Pig Model
0 Views •
2026
All animal procedures described here must be conducted in accordance with institutional animal ethics guidelines and approved by IACUC. All procedures must follow the principles of the 3Rs—Replacement, Reduction, and Refinement—and must be performed by trained personnel. Blood collection is a common procedure in swine research and clinical studies. The choice of sampling site depends on the animal's age, body size, the volume of blood required, the study objectives and approved IACUC...
06:03
Isolation and Characterization of Microvesicles from Peripheral Blood
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Cited by 74 •
2017
Extracellular vesicles present in blood have been suggested as novel biomarkers for various diseases. Here, we present a protocol for the isolation of large plasma membrane-derived microvesicles from peripheral blood samples and their subsequent analysis by conventional flow cytometry and Western Blotting.
FAQs
The collection tube condition determines whether the sample yields plasma, serum, or cellular fractions. Anticoagulants prevent clot formation when plasma or intact blood cells are needed, whereas clot-activating conditions support serum preparation. Selecting the appropriate condition is therefore essential because the resulting fraction must match the intended analysis, such as immune profiling, serology, or nucleic acid testing.
Careful handling and transport help preserve leukocytes, antibodies, nucleic acids, and other analytes after the sample leaves the vessel. Poor control during these stages can compromise the material needed for downstream laboratory analysis. Maintaining sample integrity is especially important when researchers examine immune responses, detect pathogens, or compare specimens collected at different time points.
A properly collected specimen can provide cellular fractions for examining leukocytes and molecular material for nucleic acid analysis, while serum or plasma can support antibody-focused testing. This range allows one collection approach to contribute to complementary measurements of immune status and infection. The selected tube condition and subsequent handling determine which of these analytes remains suitable for study.
The basic workflow includes disinfecting the skin, accessing a peripheral vessel with a sterile needle, transferring the blood into appropriately selected tubes, labeling each tube, and managing the sample for laboratory analysis. These steps connect patient identification and specimen quality with the requirements of the planned assay. Consistency across the workflow improves the reliability of downstream results.
Researchers use these specimens for immune profiling, pathogen detection, serology, hematology, and evaluation of responses to infection or vaccination. The same collection method can therefore support studies of immune cells, antibodies, blood characteristics, and infection-related signals. Its value lies in providing circulating material that can be matched to the specific laboratory question and analytical fraction required.
Serial blood samples can provide material for comparing immune or infection-related measurements over the course of a study. Depending on the selected fraction, investigators may examine leukocytes, antibodies, nucleic acids, or other analytes. This supports assessment of changes associated with infection or vaccination, provided collection, labeling, handling, and transport preserve comparable sample quality across time points.