Salmonella Typhimurium
Salmonella Typhimurium - Related Videos
02:41
Preparation of Salmonella typhimurium Culture
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2025
Source: Gutiérrez, S., et. al., Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages. J. Vis. Exp. (2017)This video demonstrates the culturing and preparation of Salmonella typhimurium to obtain a clean, metabolically active bacterial suspension suitable for experimental use.
06:50
A Protocol to Infect Caenorhabditis elegans with Salmonella typhimurium
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Cited by 1 •
2014
C. elegans has emerged as a new genetic model to study host-pathogen interactions. Here we describe a protocol to infect C. elegans with Salmonella typhimurium coupled with the double-strand RNAi interference technique to examine the role of host genes in defense against Salmonella infection.
01:33
Anti-Tumor Effect of a Fusion Peptide Delivered by Engineered Salmonella typhimurium in a Mouse Model
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2026
Begin with mice bearing solid tumors implanted subcutaneously in the thigh.Inject the mice via the tail vein with a non-virulent, engineered strain of Salmonella typhimurium.The bacteria circulate in the bloodstream and selectively accumulate in the hypoxic, immunosuppressed tumor microenvironment.They carry a plasmid encoding a therapeutic fusion peptide composed of a cell-penetrating peptide and the mitochondrial target domain of a pro-apoptotic protein, along with phage-derived lysis...
10:33
Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages
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Cited by 5 •
2017
We describe here a simple and quick method for the isolation of Salmonella typhimurium-containing phagosomes from macrophages by coating the bacteria with biotin and streptavidin.
12:18
An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium
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Cited by 2 •
2011
We describe a multiplex PCR for the rapid detection of Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium. Specific Salmonella serovars can be identified by targeting a multiplex PCR to genes and sequences unique to the O-antigen biosynthesis cluster and flagellin of a given serovar. Serovar is assigned then to a Salmonella isolate based on the appearance of specific, size amplicons (PCR product) corresponding to the target allele.
FAQs
Salmonella Typhimurium uses the type III secretion system encoded by Salmonella pathogenicity island 1 to promote invasion of intestinal epithelial cells. This system is linked to the early intestinal stage of infection, helping explain how exposure after ingestion can progress from contact with the gut lining to host-cell entry. Its activity is central to studying bacterial invasion mechanisms.
Pathogenicity island 2 encodes a second type III secretion system with a different functional emphasis: supporting bacterial survival and replication inside host cells, including macrophages. The contrast with pathogenicity island 1 helps researchers distinguish entry into intestinal epithelial cells from persistence in intracellular environments. This division of function provides a framework for analyzing infection after initial invasion.
Bacterial activities can trigger innate immune recognition and inflammatory signaling after infection. These responses promote recruitment of immune cells to the intestine, linking molecular detection to tissue-level defense. Studying this sequence helps immunologists examine protective host responses while also investigating the inflammation associated with Salmonella Typhimurium gastroenteritis and the ways bacterial infection influences intestinal tissues.
Intestinal epithelial cells and macrophages represent distinct host-cell contexts in the infection process. Salmonella Typhimurium uses its pathogenicity island 1 system to invade epithelial cells, whereas its pathogenicity island 2 system supports survival and replication within macrophages. Comparing these contexts helps researchers connect initial tissue invasion with intracellular persistence and immune evasion.
A conceptual infection sequence begins with ingestion, followed by bacterial interaction with and invasion of intestinal epithelial cells through the pathogenicity island 1 system. Subsequent analysis can address survival and replication within host cells through pathogenicity island 2, alongside innate immune recognition, inflammatory signaling, and immune-cell recruitment. This sequence organizes bacterial and host events over the course of infection.
Salmonella Typhimurium provides a model that connects bacterial invasion, intracellular survival, immune evasion, and host defense within one infection context. Researchers can use it to examine how pathogen activities shape innate immune responses and intestinal inflammation while studying mechanisms relevant to gastroenteritis. This broad scope makes the organism useful for linking microbial behavior with immunological outcomes.
Research on Salmonella Typhimurium can support vaccine and antimicrobial strategy development by clarifying how invasion, intracellular survival, immune evasion, and host inflammation are connected. In immunology, the model links bacterial mechanisms to defense responses. In infection research, it helps identify biological processes that may be targeted to reduce disease or limit bacterial persistence.