Tissue Seal
Tissue Seal - Related Videos
04:41
Optimized Sealing Process and Real-Time Monitoring of Glass-to-Metal Seal Structures
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Cited by 7 •
2019
Key procedures to optimize the sealing process and achieve real-time monitoring of the metal-to-glass seal (MTGS) structure are described in detail. The embedded fiber Bragg grating (FBG) sensor is designed to achieve online monitoring of temperature and high-level residual stress in the MTGS with simultaneous environmental pressure monitoring.
06:10
Accessible Silicone Chip-to-Membrane Sealing Procedure for Flexible, Reliable Bonding
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2026
A novel sealing protocol to produce water-tight silicone-based seals for membranes of Polydimethylsiloxane (PDMS) chip devices. Intended for labs setting-up chip models and small-scale chip platform development. We demonstrate the protocol using plastic and native tissue membranes. This procedure only requires PDMS, toluene, mold, membrane, and a vacuum chamber.
07:51
The C-seal: A Biofragmentable Drain Protecting the Stapled Colorectal Anastomosis from Leakage
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Cited by 8 •
2010
The C-seal is a biofragmentable drain protecting the stapled colorectal anastomosis. In this video, details of the manufacturing, deployment and use are shown.
06:26
Achieving Moderate Pressures in Sealed Vessels Using Dry Ice As a Solid CO2 Source
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Cited by 11 •
2018
Here we present a protocol for performing reactions in simple reaction vessels under low-to-moderate pressures of CO2. The reactions can be performed in a variety of vessels simply by administering the carbon dioxide in the form of dry ice, without the need for costly or elaborate equipment or set-ups.
07:48
Working with Human Tissues for Translational Cancer Research
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Cited by 4 •
2015
Translational cancer research is dependent on extraction of human tissues. Much work has gone into optimizing extraction methods for ex vivo analysis. Here, we describe tissue processing methods allowing for maximal data output from limited samples.
FAQs
Cell-cell junctions create physical connections between neighboring cells, while coordinated cytoskeletal contraction helps draw those cells into a more continuous barrier. Extracellular matrix interactions provide additional attachment and structural support. Together, these mechanisms determine how effectively a damaged epithelial surface closes and whether it can recover barrier integrity rather than remain vulnerable to leakage or microbial passage.
Extracellular matrix interactions help cells attach to and organize the material surrounding them during closure. This support complements cell-cell junctions, which connect adjacent cells, and cytoskeletal contraction, which changes cell shape and tension. Considering all three components is important because a seal must remain mechanically connected while the tissue reorganizes after damage.
After injury, platelets and inflammatory signals help organize the repair response rather than serving only as secondary reactions. Their activity coordinates events that support closure and restoration of the damaged barrier. In immunology and infection research, this connection matters because repair signals operate alongside junctional restrictions that limit the opportunity for microbes to cross exposed tissue.
Junctional barriers reduce the open pathways between cells, making it harder for microbes to pass through damaged or exposed epithelial and mucosal surfaces. This physical restriction works with ongoing repair processes to preserve barrier function. When closure is effective, the tissue is better positioned to prevent infection; when it is disrupted, microbial access becomes more likely.
Useful outcomes include reduced fluid loss, renewed barrier integrity, and a more continuous connection among cells or across damaged tissue. Researchers can also consider whether the repaired surface limits microbial passage. These outcomes connect structural closure with biological performance, showing whether repair has merely covered an injury or has restored meaningful protective function.
Incomplete or poorly maintained closure can leave epithelial or mucosal barriers permissive to pathogen entry. Continued exposure may then coincide with persistent inflammatory activity, linking barrier failure to chronic inflammation. Studying tissue sealing therefore helps connect local repair defects with broader infection-related outcomes, including loss of protection and failure to regain normal tissue function.