Pulmonary Vein Isolation

Pulmonary vein isolation (PVI) is a catheter-based cardiac ablation procedure that electrically separates the pulmonary veins from the left atrium, primarily to reduce recurrent atrial fibrillation. During the procedure, clinicians create a continuous ring of lesions around the pulmonary vein openings using thermal energy, such as radiofrequency or cryothermal ablation, which blocks abnormal electrical impulses from entering the atrium. PVI is an important rhythm-control treatment for selected patients with symptomatic atrial fibrillation, and it can lessen arrhythmia burden, improve quality of life, and reduce reliance on antiarrhythmic drugs. Its effectiveness depends on durable isolation and patient-specific disease characteristics.

Pulmonary Vein Isolation - Related Videos

Research

JoVE Journal - Medicine

Catheter-based Endovascular Angioplasty for Fibrosing Mediastinitis-associated Pulmonary Vein Stenosis

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2025

Pulmonary vein stenosis (PVS) is a life-threatening complication of fibrosing mediastinitis (FM), with a poor prognosis if not properly treated. Currently, catheter-based endovascular angioplasty is a promising therapeutic strategy for FM-associated PVS. This protocol primarily outlines a stepwise approach to the transcatheter procedure for FM-associated PVS.

Research

JoVE Journal - Medicine
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Direct Pressure Monitoring Accurately Predicts Pulmonary Vein Occlusion During Cryoballoon Ablation

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Cited by 18 •

2013

Effective pulmonary vein isolation utilizing a cryoballoon depends on complete pulmonary vein occlusion. The point of occlusion can be effectively predicted by direct analysis of pulmonary vein pressure waveform analysis during balloon inflation using a simple and reproducible technique.

Seldinger Technique Based Pulmonary Artery Catheterization: A Procedure to Insert Pulmonary Artery Catheter Through the Jugular Vein in a Porcine Model

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2025

In this video, we have demonstrated a step-by-step procedure of pulmonary artery catheterization by inserting an intravascular catheter through jugular vein and reach the pulmonary artery to measure the cardiac chamber pressure in porcine model.

Pressure Fixation of Isolated Murine Pulmonary Valve: A Procedure to Isolate and Chemically Fix the Mouse Pulmonary Valve in Closed Conformation

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2025

In this video, we describe the procedure to isolate and chemically fix the mouse pulmonary value in closed conformation via perfusion pressurization.

Isolation of Human Umbilical Vein Endothelial Cells (HUVEC)

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Cited by 131 •

2007

This video protocol illustrates the isolation and culture of human umbilical vein endothelial cells (HUVEC) from human umbilical cord. Once isolated these cells can be used for in vitro angiogenesis assays like the Optimized Fibrin Gel Bead Assay also demonstrated by the Hughes lab.

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