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JoVE Journal
Immunology and Infection

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Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay

Article DOI: 10.3791/2889-v • 07:23 min • June 9th, 2011
June 9th, 2011 •
1, 1, 1, 1, 1
1Center for Vector Biology and Zoonotic Diseases, Department of Environmental Sciences, The Connecticut Agricultural Experiment Station

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We describe a method to process and screen field-collected mosquitoes for a diversity of viruses by Vero cell culture assay. By employing this technique, we have detected 9 different viruses from 4 taxonomic families in mosquitoes collected in Connecticut.

Tags

Infectious Virus Field-collected Mosquitoes Vero Cell Culture Assay West Nile Virus Dengue Virus Chickungunya Virus Surveillance And Control Programs Viral Infection Trap Location Species Grouping Pooled Specimens Buffered Saline Mixer-mill Vero Cell Cultures Cytopathic Effect Diagnostic Assays Virus Isolation Connecticut USA Human Disease New Records
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