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Journal / Biology / Isolering af ribo Bundet Gryende polypeptider
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Isolering af ribo Bundet Gryende polypeptider

Article DOI: 10.3791/4026-v • 10:15 min • July 6th, 2012
July 6th, 2012 •
1, 1
1Center for Gene Regulation in Health and Disease, Department of Biological, Geological and Environmental Sciences, Cleveland State University

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En teknik til at identificere translationelle pause-steder på mRNA, er beskrevet. Denne procedure er baseret på isolering af nascente polypeptider akkumuleres på ribosomer under in vitro-translation af et mål-mRNA, efterfulgt af størrelses-analyse af nascente kæder under anvendelse af en denaturerende gelelektroforese.

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Genetik Molecular Biology ribosomet Nascent polypeptid Co-translationel proteinfoldning synonymt codonanvendelsen genregulering
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