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Análise in vitro de PDZ-dependentes Complexos Macromoleculares CFTR Sinalização

Article DOI: 10.3791/4091-v • 10:05 min • August 13th, 2012
August 13th, 2012 •
1, 1, 1,2,3
1Department of Biochemistry & Molecular Biology, Wayne State University School of Medicine, 2Cardiovascular Research Institute, Wayne State University School of Medicine, 3Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine

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A fibrose cística regulador da condutância transmembranar (CFTR), um canal de cloreto epitelial, tem sido relatado para interagir com várias proteínas e regulam importantes processos celulares, entre eles os CFTR PDZ motivo mediadas-interacções têm sido bem documentados. Este protocolo descreve métodos desenvolvidos para montar um macromolecular CFTR PDZ-dependente sinalização complexo

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Bioquímica Edição 66 Biologia Molecular Química CFTR complexo macromolecular a interação de proteínas PDZ proteína andaime células epiteliais fibrose cística
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