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Journal / Biology / CD146の単離
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JoVE Journal Biology
CD146の単離
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CD146の単離

Article DOI: 10.3791/53782-v • 09:47 min • June 17th, 2016
June 17th, 2016 •
1,2, 1,3,4, 1,2,5
1Sinclair Centre for Regenerative Medicine, Ottawa Hospital Research Institute, 2University of Ottawa, 3Department of Neonatology and Pediatric Critical Care Medicine, Medical Faculty and University Hospital Carl Gustav Carus, Technische Universität Dresden, 4DFG Research Center and Cluster of Excellence for Regenerative Therapies (CRTD), Technische Universität, Dresden, 5Children's Hospital of Eastern Ontario Research Institute

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このプロトコルは、酵素消化、密度勾配分離、プラスチック接着およびCD146 +磁気ビーズ選択を使用することにより、ラットから原発性肺常駐間葉系間質細胞を得るための分離技術について説明します。

Tags

細胞生物学、問題108、間葉系間質細胞、組織常駐幹/前駆細胞、肺、ラット、再生医療、磁気ビーズ選択幹
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