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Journal / Immunology and Infection / Experimentell infektion med
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Experimentell infektion med
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Experimentell infektion med

Article DOI: 10.3791/54554-v • 10:10 min • November 16th, 2016
November 16th, 2016 •
*1, *1, *1, 1, 1,2,3
1Department of Immunology, University of Toronto, 2Toronto General Research Institute, University Health Network, 3Women's College Research Institute
* These authors contributed equally

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Detta protokoll beskriver hur man ympa C57BL / 6J-möss med EGD stam av Listeria monocytogenes (L. monocytogenes) och för att mäta interferon-γ (IFN-γ) svar genom naturliga mördarceller (NK), naturliga mördar-T (NKT-celler), och adaptiv T-lymfocyter efter infektion. Detta protokoll beskriver också hur man genomför studier överlevnads i möss efter infektion med en modifierad LD 50 dosen av patogenen.

Tags

Infektion , Bakterier infektionsmodell möss flödescytometri interferon-γ svar
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