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Invasive Behavior of Human Breast Cancer Cells in Embryonic Zebrafish
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Invasive Behavior of Human Breast Cancer Cells in Embryonic Zebrafish
DOI:

09:22 min

April 25, 2017

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Kapitel

  • 00:05Titel
  • 01:28Preparation of Fluorescent Cells
  • 02:42Preparation of Zebrafish Embryos
  • 03:20Xenotransplantation into the Perivitelline Space
  • 05:07Xenotransplantation into the Duct of Cuvier
  • 05:47Invasion and Metastasis Assessment
  • 06:32Results: Xenotransplanted MDA-MB-231, MCF-10A, and MCF-10A-Ras Cells Reveal Distinct Invasiveness Patterns
  • 08:17Conclusion

Summary

Automatische Übersetzung

Here, we describe xenograft zebrafish models using two different injection sites, i.e., perivitelline space and duct of Cuvier, to investigate the invasive behavior and to assess the intravasation and extravasation potential of human breast cancer cells, respectively.

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