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Journal / Genetics / Laser Microirradiation att studera In Vivo…
JoVE Journal
Genetics

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Laser Microirradiation att studera In Vivo cellulära svar till enkla och komplexa DNA-skador
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Laser Microirradiation att studera In Vivo cellulära svar till enkla och komplexa DNA-skador

Article DOI: 10.3791/56213-v • 10:44 min • January 31st, 2018
January 31st, 2018 •
*1, *2, 2, 2, 2, 2,3,4, 1
1Department of Biological Chemistry, School of Medicine, University of California, Irvine, 2Beckman Laser Institute and Medical Clinic, University of California, Irvine, 3Department of Developmental and Cell Biology, School of Biological Sciences, University of California, Irvine, 4Department of Biomedical Engineering and Surgery, University of California, Irvine
* These authors contributed equally

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Målet med detta protokoll är att beskriva hur man använder laser microirradiation att framkalla olika typer av DNA-skador, inklusive relativt enkla strand raster och komplex skada, att studera DNA skador signalering och reparation faktor församlingen vid skada platser i vivo .

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Genetik fråga 131 Laser microirradiation lasrar i medicin DNA-skador DNA skador svar DNA-reparation poly (ADP-ribos) polymeras (PARP) komplex skada strand raster bas skador
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