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Biochemistry

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Um Western que mancha o protocolo para o pequeno número de células-tronco hematopoiéticas

Article DOI: 10.3791/56855-v • 08:45 min • August 22nd, 2018
August 22nd, 2018 •
1,2,3, 1, 2
1Division of Experimental Hematology, Cincinnati Children's Hospital Medical Center, 2Department of Cell and Developmental Biology, Abramson Family Cancer Research Institute, Perelman School of Medicine, University of Pennsylvania, 3Department of Obstetrics and Gynecology, Southwest Hospital, Third Military Medical University

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Um padrão ocidental que mancha o protocolo foi otimizado para analisar apenas 500 tronco hematopoiético ou células progenitoras. Otimização envolve cuidado, manipulação da amostra celular, limitando as transferências entre os tubos e lise diretamente as células no buffer de Laemmli sample.

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Bioquímica edição 138 mancha ocidental células-tronco hematopoiéticas número de células pequenas SDS-PAGE reduzir a experimentação animal medula óssea
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