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Journal / Genetics / 準備とヒト以外の霊長造血幹・前駆細胞の遺伝子の改変…
JoVE Journal
Genetics

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JoVE Journal Genetics
準備とヒト以外の霊長造血幹・前駆細胞の遺伝子の改変
Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform
Journal (Genetics)
Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform
A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
Journal (Genetics)
A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
 
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準備とヒト以外の霊長造血幹・前駆細胞の遺伝子の改変

Article DOI: 10.3791/58933-v • 11:16 min • February 15th, 2019
February 15th, 2019 •
1, 1, 1, 1, 1, 1, 1,2,3, 1,2
1Stem Cell and Gene Therapy Program, Fred Hutchinson Cancer Research Center, 2Department of Medicine, University of Washington, 3Department of Pathology, University of Washington

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このプロトコルの目標はヒト以外の霊長類 CD34 を分離、+細胞プライミング骨髄、レンチ ウイルスのベクトルでこれらの細胞の遺伝子変更および自己ホストの注入の製品を準備します。総プロトコルの長さは、約 48 時間です。

Tags

遺伝学、問題 144、造血幹前駆細胞、遺伝子治療、造血、ヒト以外の霊長類モデル、細胞分離、レンチウイルスベクター
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