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Gebruik van primaire gekweekte hippocampale neuronen om de assemblage van axon-initiële segmenten te bestuderen

Article DOI: 10.3791/61411-v • 06:53 min • February 12th, 2021
February 12th, 2021 •
1,2,3, 2,3
1School of Basic Medical Sciences, Xi'an Jiaotong University, 2Department of Cell Biology, Duke University, 3Department of Biochemistry, Duke University

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Hier beschreven we een protocol om de assemblage en structuur van de axon initiële segmenten (AIS) van hippocampale neuronen die geen vooraf geassembleerde AIS missen vanwege de afwezigheid van een gigantische ankyrine-G kwantitatief te bestuderen.

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Retractie Giant ankyrin-G Axon initial segment Primary cultured neurons Transfection Imaging AIS Intensity quantification
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