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Immunology and Infection

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JoVE Journal Immunology and Infection
Purification et expansion de cellules T tueuses naturelles invariantes de souris pour des études in vitro et in vivo
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Purification et expansion de cellules T tueuses naturelles invariantes de souris pour des études in vitro et in vivo

Article DOI: 10.3791/62214-v • 08:37 min • February 15th, 2021
February 15th, 2021 •
1, *1,2, *1,2, 1,2
1Experimental Immunology Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, 2Vita-Salute San Raffaele University
* These authors contributed equally

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Summary
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Nous décrivons un protocole rapide et robuste pour enrichir les cellules T tueuses naturelles invariantes (iNKT) de la rate de souris et les étendre in vitro à des nombres appropriés pour des études in vitro et in vivo.

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Immunologie et infection numéro 168 cellules iNKT expansion souris Vα14 CD1d perles anti-CD3/CD28 activation lymphocytes
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