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Journal / Immunology and Infection / Analys av SAMHD1-begränsning genom…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Analys av SAMHD1-begränsning genom flödescytometri i humana myeloida U937-celler
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Analys av SAMHD1-begränsning genom flödescytometri i humana myeloida U937-celler

Article DOI: 10.3791/62502-v • 09:43 min • June 13th, 2021
June 13th, 2021 •
1, 2, 1, 3
1Retrovirus-Host Interactions Laboratory, The Francis Crick Institute, 2Retroviral Replication Laboratory, The Francis Crick Institute, 3Sidney Sussex College, Department of Medicine, University of Cambridge

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Här beskrivs en etablerad metod för att bestämma omfattningen av HIV-1-begränsning av det cellulära hämmande proteinet SAMHD1. Mänskliga myeloida U937-celler transduceras med en SAMHD1-uttrycksvektor som uttrycker YFP, differentieras och utmanas sedan med HIV-RFP. Restriktionsnivån bestäms genom flödescytometrianalys.

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Immunologi och infektion utgåva 172 humant immunbristvirus typ 1 (HIV-1) SAMHD1 begränsning myeloida celler flödescytometri retrovirus
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