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Journal / Genetics / Kromosomscreening av humane…
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Genetics

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Kromosomscreening av humane preimplantasjonsembryoer ved bruk av brukt kulturmedium
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Kromosomscreening av humane preimplantasjonsembryoer ved bruk av brukt kulturmedium: prøveinnsamling og kromosomal ploidianalyse

Article DOI: 10.3791/62619-v • 12:32 min • September 7th, 2021
September 7th, 2021 •
*1,2,3,4, *5, 1,2,3,4, 1,2,3,4, 5, 5, 1,2,3,4, 5
1Centre for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, 2National Clinical Research Center for Obstetrics and Gynecology(Peking University Third Hospital), 3Ministry of Education, Key Laboratory of Assisted Reproduction (Peking University), 4Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, 5Department of Clinical Research, Yikon Genomics Co. Ltd.
* These authors contributed equally

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Denne studien rapporterer en protokoll for kromosomscreening av humane embryoer som bruker brukt kulturmedium, som unngår embryobiopsi og muliggjør kromosomploidiidentifikasjon ved hjelp av NGS. Denne artikkelen presenterer den detaljerte prosedyren, inkludert utarbeidelse av kulturmedium, hele genomamplifikasjon (WGA), neste generasjons sekvensering (NGS) bibliotekforberedelse og dataanalyse.

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Kromosomscreening embryoer med menneskelig preimplantasjon brukt kulturmedium prøvetaking kromosomal ploidianalyse in vitro-fertilisering (IVF) preimplantasjonsgenetisk testing for aneuploidi (PGT-A) trohektoderm (TE) indre cellemasse (ICM) falske positive og negativer NICS-teknologi DNA-sekvensering kulturmediumprøvetaking helgenomamplifisering (WGA) bibliotekforberedelse NGS-dataanalyse kryopreservering IVF-laboratorium
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