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Journal / Cancer Research / In Vivo Immunogenisitet Screening av…
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In Vivo Immunogenisitet Screening av tumor-avledede ekstracellulære vesicles av Flow Cytometry av splenic T-celler
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In Vivo Immunogenisitet Screening av tumor-avledede ekstracellulære vesicles av Flow Cytometry av splenic T-celler

Article DOI: 10.3791/62811-v • 08:02 min • September 23rd, 2021
September 23rd, 2021 •
1,2, 1,2,3,4, 1,2
1Department of Medicine III, School of Medicine, Technical University of Munich, 2Center for Translational Cancer Research (TranslaTUM), School of Medicine, Technical University of Munich, 3Department of Internal Medicine III, University Hospital Regensburg, 4National Centre for Tumor Diseases WERA

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Dette manuskriptet beskriver hvordan man vurderer in vivo immunogenisitet av tumorcelleavledede ekstracellulære vesyrer (EVs) ved hjelp av strømningscytometri. Elbiler avledet fra svulster som gjennomgår behandlingsindusert immunogen celledød virker spesielt relevante i tumorimmunosurveillans. Denne protokollen eksemplifiserer vurderingen av oksaliplatininduserte immunostimulatoriske tumor-elbiler, men kan tilpasses ulike innstillinger.

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