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Journal / Bioengineering / Y-27632를 사용하여 1차 인간 칭기발 상피 세포의 격리 및…
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Bioengineering
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Y-27632를 사용하여 1차 인간 칭기발 상피 세포의 격리 및 배양

Article DOI: 10.3791/62978-v • 06:47 min • November 6th, 2021
November 6th, 2021 •
1,2,3, 4, 3, 1, 1, 1, 1, 1, 1, 2,5, 1
1Department of Tissue Engineering and Regeneration, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, 2Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, 3Department of Stomatology, Shengli Oilfield Central Hospital, 4Department of Pediatric Surgery, Shengli Oilfield Central Hospital, 5Ningbo Stomatology Hospital of Savaid Stomatology School, Hangzhou Medical College

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여기서 우리는 전통적인 방법에 록 억제제 Y-27632를 추가하여 인간 칭기발 상피 세포의 격리 및 배양에 대한 변형된 방법을 제시한다. 이 방법은 더 쉽고 시간이 많이 걸리며 줄기 세포 특성을 향상시키며 실험실과 임상 응용 분야에서 더 많은 수의 고잠재 상피 세포를 생성합니다.

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생명공학 제177호 칭지발 상피세포 효소방법 직접 절제방법 세포 격리 세포배양 Y-27632 로관련 키나제 억제제 면역형광분석
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