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Flödescytometrisk analys av biomarkörer för att upptäcka funktionella defekter hos mänskliga spermier
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Flödescytometrisk analys av biomarkörer för att upptäcka funktionella defekter hos mänskliga spermier

Article DOI: 10.3791/63790-v • 08:48 min • April 21st, 2022
April 21st, 2022 •
1, 1, 1, 1, 2, 1, 1, 1, 1, 1
1Institute of Toxicology, College of Preventive Medicine, Army Medical University (Third Military Medical University), 2Department of Chemical Defense Medicine, College of Military Preventive Medicine, Army Medical University (Third Military Medical University)

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Det nuvarande protokollet ger en praktisk lösning som möjliggör mätning av apoptos, mitokondriell membranpotential och DNA-skador i mänskliga spermier med en enda cytometer.

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Flödescytometri biomarkörer spermiefunktionsdefekter konventionella spermaparametrar idiopatisk infertilitet spermieapoptos mitokondriell membranpotential (MMP) DNA-skador FCM-tekniker annexin V-fluoresceinisotiocyanat (FITC)/propidiumjodid (PI) färgning 5,5 & 8242; ,6 & 8242; -tetraklor-1,1 & 8242;,3 & 8242;-tetraetylbensimidazolylkarbocyanin jodid (JC-1) sond spermiekromatinstrukturanalys (SCSA) akridinorange (AO) färgning
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