Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Et "dual-addition" calciumfluorescensassay til…
JoVE Journal
Biochemistry

Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.

JoVE Journal Biochemistry
Et "dual-addition" calciumfluorescensassay til high-throughput screening af rekombinante G-proteinkoblede receptorer
Functional Characterization of Carboxylesterases in Insecticide Resistant House Flies, Musca Domestica
Journal (Biochemistry)
Functional Characterization of Carboxylesterases in Insecticide Resistant House Flies, Musca Domestica
Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies
Journal (Biochemistry)
Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies
 
Click here for the English version

Et "dual-addition" calciumfluorescensassay til high-throughput screening af rekombinante G-proteinkoblede receptorer

Article DOI: 10.3791/64505-v • 08:46 min • December 2nd, 2022
December 2nd, 2022 •
1, 2, 1
1Department of Entomology, Texas A&M University, 2Department of Biochemistry and Biophysics, Texas A&M University

Kapitel

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

I dette arbejde beskrives et højt gennemløb, intracellulært calciumfluorescensassay for 384-brøndplader til screening af små molekylebiblioteker på rekombinante G-proteinkoblede receptorer (GPCR'er). Målet, kininreceptoren fra kvægfeberflåten, Rhipicephalus microplus, udtrykkes i CHO-K1-celler. Dette assay identificerer agonister og antagonister, der bruger de samme celler i et "dual-addition" assay.

Tags

Biokemi nr. 190
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter