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Journal / Bioengineering / 미세유체 혼합으로 제조된 mRNA-지질 나노입자의 In vitro 및 In vivo 효율 …
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
미세유체 혼합으로 제조된 mRNA-지질 나노입자의 In vitro 및 In vivo 효율 테스트
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미세유체 혼합으로 제조된 mRNA-지질 나노입자의 In vitro 및 In vivo 효율 테스트

Article DOI: 10.3791/64810-v • 08:55 min • January 20th, 2023
January 20th, 2023 •
1, 1, 1, 1, 1
1Department of Bioengineering, University of Pennsylvania

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여기에서, 반딧불이 루시페라아제를 인코딩하는 mRNA를 캡슐화하는 지질 나노입자(LNP)를 제형화하기 위한 프로토콜이 제시된다. 이 LNP는 HepG2 세포의 in vitro 및 C57BL/6 마우스의 in vivo 에서 효능을 테스트했습니다.

Tags

생명공학 191호 In vitro Efficiency In Vivo Efficiency LNPs mRNA Cargo Nucleases 분해 Intracellular delivery Ionizable Lipid Phospholipid Cholesterol Lipid-PEG Conjugate Firefly Luciferase Transfection Efficiency HepG2 Cells Bioluminescent Plate-based Assay C57BL/6 Mice Intravenous Injection Lateral Tail Vein Whole-body Bioluminescence Imaging In Vivo Imaging System
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