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Journal / Developmental Biology / Differentiering av humana pluripotenta stamceller …
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Developmental Biology

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Differentiering av humana pluripotenta stamceller till insulinproducerande ö-kluster
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Differentiering av humana pluripotenta stamceller till insulinproducerande ö-kluster

Article DOI: 10.3791/64840-v • 08:41 min • June 23rd, 2023
June 23rd, 2023 •
*1, *1, 1, 1, 1,2, 3, 1,2,4
1Department of Cellular & Physiological Sciences, Life Science Institute, University of British Columbia, 2School of Biomedical Engineering, University of British Columbia, 3STEMCELL Technologies Inc, 4Department of Surgery, University of British Columbia
* These authors contributed equally

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Differentieringen av stamceller till ö-celler ger en alternativ lösning till konventionell diabetesbehandling och sjukdomsmodellering. Vi beskriver ett detaljerat stamcellsodlingsprotokoll som kombinerar ett kommersiellt differentieringskit med en tidigare validerad metod för att hjälpa till att producera insulinfrisättande, stamcellshärledda öar i en maträtt.

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Utvecklingsbiologi utgåva 196 Insulinproducerande ökluster betaceller diabetesbehandling protokoll pankreasstamcellskit R-protokoll mikrobrunnsplattor endokrin differentiering 96-brunnars statiskt suspensionsformat in vitro-karakterisering funktionell utvärdering HPSC-härledda öar stamcellsodlingstekniker biologiska analyser
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