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Scalable Transfection of Maize Mesophyll Protoplasts
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Scalable Transfection of Maize Mesophyll Protoplasts

Article DOI: 10.3791/64991-v • 08:38 min • June 23rd, 2023
June 23rd, 2023 •
1, 1, 1, 1, 1
1Genome Sciences Department, University of Washington

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Summary
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Here, we present a high-throughput protocol to transiently transfect millions of maize protoplasts for testing large libraries of plasmids in maize mesophyll cells.

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Scalable Transfection Maize Mesophyll Protoplasts Protocol Plant Cell Wall Digestion PEG Transformation High Efficiency Millions Of Transformed Protoplasts Maize Seedlings Dark Growth Leaf Selection Leaf Cutting Consistent Thin Slices Enzyme Solution
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