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Chemistry

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Chemistry

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Analytical Chemistry

Ion-Exchange Chromatography for Sample Cleanup

Description

Ion-exchange chromatography is a separation method that cleans up samples by using charge. A column is packed with an ion-exchange resin, which is a charged stationary phase on a solid support. Strong cation-exchange chromatography uses a negatively charged resin to separate cations. Strong anion-exchange chromatography uses...

Transcript

La cromatografía de intercambio iónico se usa ampliamente en la separación y aislamiento de compuestos cargados, particularmente biomoléculas grandes.

Este tipo de cromatografía líquida utiliza una columna de perlas de fase estacionaria empaquetadas, llamadas resina. La técnica separa los analitos en función de su afinidad con la resina c...

Tags

Cation Exchange ChromatographyAnion Exchange ChromatographyProtein SeparationSalt Gradient ElutionColumn EquilibrationBuffer pH SelectionHydroxyapatite ChromatographyImmobilized Metal Affinity ChromatographyHigh Performance Liquid Chromatography

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