Name: atomic emission spectroscopy for element analysis
Uploaded: 2024-12-05T18:32:15.000Z
Duration: 1 min 20 s
Channel: JoVE

K12 Standards

Pennsylvania State Academic Standards

9-12

Earth and Space Science

Space Systems

3.3.9-12.C

Atomic Emission Spectroscopy: Overview

La espectroscopia de emisión atómica, o AES, permite el análisis multielemental en una muestra, donde todos los analitos pueden ser excitados simultáneamente por descarga eléctrica, llama o plasma.
En AES, los átomos de estado excitado se relajan para dar emisiones características, y la intensidad de la emisión es directamente proporcional a la población de estado excitado.
Se logran poblaciones significativas de átomos en estado excitado mediante el empleo de plasma de argón a alta temperatura.
El plasma de argón, una mezcla gaseosa caliente y conductora de electricidad de iones de argón y electrones, se genera ionizando una corriente de argón que fluye. El plasma se mantiene indefinidamente gracias a una fuente de alimentación que mantiene una temperatura óptima para una mayor ionización. 
Una fuente de plasma basada en radiofrecuencia llamada plasma acoplado inductivamente, o ICP, puede alcanzar temperaturas de hasta 10.000 K. Es una fuente de excitación ampliamente utilizada en AES, comúnmente conocida como ICP-AES o ICP-OES.
Otras fuentes de plasma incluyen plasma inducido por microondas, plasma de corriente continua, microplasmas y plasma inducido por láser.

espectroscopia de emisión atómica: descripción general

La espectroscopia de emisión atómica (AES) es una técnica analítica que se utiliza para determinar la composición elemental de una muestra mediante el análisis de la luz emitida por átomos excitados. En la AES, los átomos de una muestra se excitan a niveles de energía más altos mediante energía térmica de fuentes de alta temperatura, como plasma, arcos o chispas. Cuando estos átomos excitados regresan a estados de energía más bajos, emiten luz en longitudes de onda específicas características de cada elemento. El espectro de emisión atómica resultante, que consta de líneas discretas que corresponden a estas longitudes de onda, permite identificar y cuantificar varios elementos dentro de la muestra.

La instrumentación AES comparte similitudes con los espectrómetros de absorción atómica, pero con adaptaciones específicas para la detección de emisiones. Las fuentes de alta temperatura, en particular el plasma acoplado inductivamente (ICP), son esenciales en la AES, ya que alcanzan la energía suficiente para excitar los átomos a sus estados de emisión. Otras fuentes de plasma incluyen el plasma inducido por microondas (MIP) y el plasma de corriente continua (DCP). La fuente más utilizada, la ICP, alcanza temperaturas de hasta 10 000 K, lo que crea un entorno estable para una excitación y emisión constantes. La ICP-AES, también conocida como ICP-OES (espectrometría de emisión óptica), permite el análisis de múltiples elementos al colocar múltiples detectores en una matriz semicircular alrededor de la fuente de emisión para capturar lecturas simultáneas en un rango de longitudes de onda.

La AES ofrece varias ventajas sobre los métodos de absorción atómica tradicionales, como las técnicas de llama y electrotérmicas. Debido a las fuentes de alta temperatura que disocian moléculas complejas, la AES es menos susceptible a las interferencias químicas, lo que permite lecturas espectrales más limpias. La técnica permite el análisis simultáneo de múltiples elementos, lo que mejora significativamente la eficiencia analítica. Además, la AES cubre un rango de concentración más amplio, lo que la hace adecuada para diversos tipos de muestras.

A pesar de estas ventajas, la AES tiene limitaciones. Los espectros complejos producidos por fuentes de alta temperatura aumentan la probabilidad de interferencias espectrales, lo que complica el análisis cuantitativo. Para abordar estos desafíos, los instrumentos AES requieren sistemas ópticos de alta resolución, a menudo más costosos que los utilizados en la espectrometría de absorción atómica. Además, si bien la AES es potente para el análisis de múltiples elementos, las técnicas de absorción atómica siguen siendo valiosas para el análisis de un solo elemento debido a su simplicidad, rentabilidad y precisión.

La AES se utiliza ampliamente en el monitoreo ambiental, la ciencia de los materiales y los laboratorios clínicos para analizar metales, oligoelementos y otras sustancias inorgánicas. Su capacidad para realizar análisis rápidos de múltiples elementos hace que la AES sea particularmente útil para analizar muestras de suelo, agua y biológicas. La alta sensibilidad y el amplio rango elemental de los instrumentos AES permiten mediciones precisas tanto en concentraciones traza como significativas, lo que lo convierte en una herramienta versátil para el análisis elemental en varios campos científicos.

En AES, el análisis cuantitativo se basa en la medición de la intensidad de la luz emitida, que es proporcional a la población de átomos excitados. Según la distribución de Boltzmann, esta población de estado excitado depende de la temperatura de la fuente de excitación, y las temperaturas más altas producen mayores emisiones. Las curvas de calibración, a menudo lineales en varios órdenes de magnitud, se crean analizando estándares conocidos para correlacionar las intensidades de emisión con las concentraciones elementales. Las técnicas de estandarización son cruciales para controlar las variaciones en la eficiencia de excitación y otros factores instrumentales, lo que permite una cuantificación precisa de elementos en diversas muestras.

Espectroscopia de emisión atómica: descripción general

La espectroscopia de emisión atómica (AES) es una técnica analítica utilizada para determinar la composición elemental de una muestra mediante el análisis de la luz emitida por ...

Education

Educación

JoVE Core

Analytical Chemistry

Química Analítica

Unit 1

Unidad 1

Atomic Spectroscopy

Espectroscopía Atómica

KEY TERMS AND CONCEPTS

TÉRMINOS Y CONCEPTOS CLAVE

atomic emission spectroscopy for element analysis

Atomic emission spectroscopy is used to identify the elements in a sample by measuring the light they emit. Atoms are first excited to higher energy levels by thermal energy from hot sources such as plasma, arcs, or sparks. When they fall back to lower energy levels, they give off light at specific wavelengths. Each element produces its own set of lines, so the emission spectrum can show both what is present and how much is there.

AES instruments are similar to atomic absorption spectrometers, but they are built to detect emitted light. High-temperature sources are essential because they provide enough energy to excite atoms into their emission states. Inductively coupled plasma, or ICP, is the most widely used source. It can reach temperatures up to 10,000 K and creates a stable environment for steady excitation and emission. Other plasma sources used in AES include microwave-induced plasma, or MIP, and direct current plasma, or DCP.

ICP-AES is also called ICP-OES, which stands for optical emission spectrometry. This setup allows multielement analysis by placing multiple detectors in a semicircular array around the emission source. The detectors can record several wavelengths at the same time. That makes the method fast and useful when more than one element must be measured in the same sample.

AES has several advantages over traditional atomic absorption methods such as flame and electrothermal techniques. The high-temperature source helps break apart complex molecules, so chemical interferences are usually lower. The method also supports simultaneous multielement analysis, which improves efficiency. In addition, AES covers a wider concentration range, so it works with many different sample types.

The technique also has limits. High-temperature sources create complex spectra, which increases the chance of spectral interferences and can make quantitative analysis harder. AES instruments need high-resolution optical systems to separate the lines clearly, and these systems are often more expensive than those used in atomic absorption spectrometry. For single-element analysis, atomic absorption methods are still useful because they are simple, cost-effective, and precise.

AES is widely used in environmental monitoring, materials science, and clinical laboratories. It can analyze metals, trace elements, and other inorganic substances in soil, water, and biological samples. This makes it valuable for rapid testing across many scientific fields. The method is sensitive and can measure both trace and significant concentrations.

Quantitative AES depends on the intensity of the emitted light. That intensity is related to the number of atoms in the excited state. According to the Boltzmann distribution, this excited-state population depends on the temperature of the source, so higher temperatures produce more emission. To find concentration, scientists use calibration curves made from known standards, and they apply standardization methods to reduce the effects of changes in excitation efficiency and other instrument factors.

Atomic Emission Spectroscopy for Element Analysis

atomic-emission-spectroscopy-for-element-analysis

atomic spectroscopy: absorption, emission, and fluorescence

atomic-spectroscopy-absorption-emission-and-fluorescence

Element Analysis with Atomic Light Spectra

Atomic spectroscopy is used to identify and measure elements by their light signals. It supports both qualitative analysis, which tells what is present, and quantitative analysis, which tells how much is present. The optical methods in this group are atomic absorption spectroscopy, atomic emission spectroscopy, and atomic fluorescence spectroscopy. These methods begin with atomization. In this step, a solid, liquid, or solution sample is changed into gas-phase atoms and ions. Once the sample...

atomic spectroscopy: effects of temperature

atomic-spectroscopy-effects-of-temperature

How Heat Changes Atomic Spectra

Heat changes atomic spectra by affecting atomization, excitation, ionization, and line shape. In atomic spectroscopy, atomization means converting a sample into gas-phase atoms and ions. The flame temperature needed for this step changes how efficiently the method works and shifts the balance between excited and ground-state atoms. At thermal equilibrium, the relative numbers of excited and ground-state atoms can be estimated with the Maxwell-Boltzmann distribution. A small temperature rise...

atomic absorption spectroscopy: overview

Atomic Absorption Spectroscopy for Trace Metals

Atomic absorption spectroscopy (AAS) measures trace metals by tracking how atoms absorb electromagnetic radiation (EMR). The absorbed light makes atoms move to a higher-energy orbit. In AAS, the key step is atomization, which converts the sample into gas-phase atoms. A flame or a furnace is typically used for this step. Inside the flame, some atoms become thermally excited. Most stay in the ground state, which is the lowest-energy state. These ground-state gas-phase atoms absorb EMR only when...

atomic absorption spectroscopy: instrumentation

atomic-absorption-spectroscopy-instrumentation

Atomic Absorption Spectroscopy: Key Parts

Atomic absorption spectrophotometry uses a set of key parts to measure light absorbed by atoms. The main components are a radiation source, an atomizer, a monochromator, and a detector. Each part has a specific job in the instrument. The radiation source is usually a hollow-cathode lamp, or HCL, or an electrodeless-discharge lamp, or EDL. Both produce a narrow emission line at the needed wavelength. Some instruments instead use continuum sources with high-resolution monochromators to narrow...

atomic absorption spectroscopy: radiation and light sources

atomic-absorption-spectroscopy-radiation-and-light-sources

Choosing Light Sources for AAS Measurements

Atomic absorption spectroscopy (AAS) uses a light source that emits a very narrow range of wavelengths. This is needed because the source must match the absorption behavior of the analyte atom. The choice of source also depends on giving a precise, intense emission at the right wavelength for accurate detection. Two common line sources in AAS are hollow-cathode lamps (HCLs) and electrodeless-discharge lamps (EDLs). These lamps are selected based on the element being analyzed and the level of...

atomic absorption spectroscopy: atomization methods

atomic-absorption-spectroscopy-atomization-methods

Atomic Absorption Spectroscopy: Sample Atomization

Atomic absorption spectroscopy uses sample atomization to turn a liquid specimen into free atoms for analysis. The two main approaches are flame atomization and electrothermal atomization. Both methods prepare the sample so its atoms can be measured by the instrument. Flame atomization uses a nebulizer and spray chamber to mix the sample with a fuel-oxidant mixture. This creates a fine aerosol mist that enters the burner. The fuel and oxidant are usually close to a stoichiometric ratio, but a...

atomic absorption spectroscopy: interference

atomic-absorption-spectroscopy-interference

Correcting Errors in Atomic Absorption Spectroscopy

Atomic absorption spectroscopy interference can create systematic error by making the analytical signal or the background look stronger or weaker than it should be. In atomic absorption measurements, these errors are usually grouped as spectral interference, chemical interference, and physical interference. Spectral interference happens when signals from other elements or molecules overlap with the analyte signal, the substance being measured. This overlap can falsely raise or hide the analyte...

atomic absorption spectroscopy: lab

AAS Sample Prep and Calibration

Atomic absorption spectroscopy (AAS) depends on careful sample preparation and calibration. Samples must be turned into clear solutions before measurement. That often means extra treatment to dissolve materials such as soils, animal tissues, and minerals. Common sample prep methods include hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, and fusion with reagents. These steps help make the sample suitable for AAS analysis. Solutions that contain organic...

atomic emission spectroscopy: overview

Atomic emission spectroscopy is used to identify the elements in a sample by measuring the light they emit. Atoms are first excited to higher energy levels by thermal energy from hot sources such as plasma, arcs, or sparks. When they fall back to lower energy levels, they give off light at specific wavelengths. Each element produces its own set of lines, so the emission spectrum can show both what is present and how much is there. AES instruments are similar to atomic absorption spectrometers,...

atomic emission spectroscopy: instrumentation

atomic-emission-spectroscopy-instrumentation

AES Sample Handling and Detection

Atomic emission spectrometry (AES) uses sample handling, atomization, and detection parts to measure emitted light from atoms and ions. The sample must first be changed into a gas-phase form so it can produce a measurable signal. AES uses two main atomizer types. Continuous atomizers, such as flames and plasmas, feed the sample in a steady stream. Discrete atomizers add one sample at a time with syringes or autosamplers. The electrothermal atomizer is the most common discrete type. Many...

atomic emission spectroscopy: interference

atomic-emission-spectroscopy-interference

Reducing Interference in AES Signals

Atomic emission spectroscopy (AES) can produce interfering signals when atoms and molecules in a flame or plasma emit overlapping light. High-temperature atomizers excite many species at once, including oxides, hydroxides, and flame combustion products. These extra emissions can make the analyte signal harder to read. Spectral interference happens when emission lines or bands overlap. One way to reduce it is to improve instrument resolution. Another is to choose a different emission line for...

inductively coupled plasma atomic emission spectroscopy: principle

inductively-coupled-plasma-atomic-emission-spectroscopy-principle

ICP-AES Torch and Sample Analysis

Inductively coupled plasma atomic emission spectroscopy uses a plasma torch to analyze elements in a sample. This method is also called inductively coupled plasma optical emission spectroscopy, or ICP-OES. It is widely used because the plasma creates a strong environment for elemental analysis. The ICP source, or torch, contains three concentric quartz tubes. Argon gas flows through the tubes. A spark from a Tesla coil starts the ionization of argon and creates a high-temperature plasma. The...

inductively coupled plasma atomic emission spectroscopy: instrumentation

inductively-coupled-plasma-atomic-emission-spectroscopy

ICP-AES Instrument Types and Detection

Inductively coupled plasma atomic emission spectroscopy, or ICP-AES, uses an inductively coupled plasma to detect and analyze elements in a sample. The method measures the light given off by atoms, which helps identify and compare different elements. It is a common approach in atomic emission spectroscopy. ICP-AES instruments are built in three main forms: sequential, simultaneous multichannel, and Fourier transform instruments. Fourier transform designs are less commonly used, but they are...

atomic emission spectroscopy: lab

Plasma Sources in Atomic Emission Spectroscopy

Atomic emission spectroscopy uses plasma sources to identify elements by their light. The method creates abundant spectra with characteristic emission lines. Inductively coupled plasma, or ICP, is especially useful because it gives strong quantitative data. It works well under stable conditions, with low noise, low background, and few interferences. Newer air-operated microwave sources are also being studied. These sources may be more cost-effective than conventional ICP systems. Atomic...

atomic fluorescence spectroscopy

Measuring Elements by Atomic Fluorescence

Atomic fluorescence spectroscopy measures elements by detecting the light atoms give off after they are excited by electromagnetic radiation. The atoms are first placed in a flame, furnace, or plasma. They absorb energy, become excited, and then release that energy as they return to their original state. The emitted light is called fluorescence. The fluorescence is observed at a right angle to the incoming beam. This setup helps separate the emitted light from the light used to excite the...

flame photometry: overview

Flame Emission Spectrometry Basics

Flame emission spectrometry uses a flame to excite elements in a sample so their light can be measured. It is also called flame photometry. The method can be used for both qualitative and quantitative analysis of elements. The idea became established in the early 1860s when Kirchhoff and Bunsen showed that specific elements give off characteristic radiation in a flame. The first instrument for this purpose measured sodium in plant ash with a Bunsen flame. A major problem was how to add the...

flame photometry: lab

Flame Photometry and Metal Detection

Flame photometry is a method for detecting metals by measuring the light they emit in a flame. A solution such as potassium chloride is aspirated into the flame. The solvent evaporates first. The remaining salt becomes dehydrated and then dissociates into free gaseous atoms in the ground state. Some of these atoms absorb energy from the flame and move to an excited state. When they return to the ground state, they emit photons at characteristic wavelengths. These emissions are very narrow...

key terms and concepts

Learn how atomic emission spectroscopy uses emitted light to identify elements, compare ICP-AES and ICP-OES, and measure samples with calibration curves.

Atomic Emission Spectroscopy for Element Analysis | JoVE K12

analytical chemistry

JoVE Core Analytical Chemistry simplifies complex chemical principles by breaking them down into clear, engaging animations, making topics from SI Units to NMR spectroscopy more accessible and straightforward. Additionally, the scientist-in-action videos highlight the application of these concepts in real-world research conducted in laboratories globally.

Louisiana Student Standards for Science

High School

Earth Science

Earth’s Place In The Universe

HS-ESS1-2

Chemistry

motion-and-stability-forces-and-interactions

Motion And Stability: Forces And Interactions

HS-PS2-6

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South Carolina College and Career Ready Science Standards

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Waves and Their Applications in Technologies for Information Transfer

C-PS4-4

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Nuclear Stability

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3.3.9-12.A

Emission Spectra

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Nuclear Binding Energy

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Metal Flame Emission

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Atomic Emission Spectroscopy: Instrumentation

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Atomic Absorption Spectroscopy: Radiation and Light Sources

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3.3.9-12.B

Spectrophotometry: Introduction

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Atomic Emission Spectroscopy: Lab

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3.3.9-12.D

Atomic Emission Spectroscopy for Element Analysis

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