Name: plasma sources in atomic emission spectroscopy
Uploaded: 2024-12-05T18:32:16.000Z
Duration: 1 min 29 s
Channel: JoVE

K12 Standards

Pennsylvania State Academic Standards

9-12

Earth and Space Science

Space Systems

3.3.9-12.C

Atomic Emission Spectroscopy: Lab

ICP es una fuente preferida para AES debido a su alta estabilidad, bajo ruido y falta de interferencia en condiciones experimentales precisas.
Por lo general, analiza muestras de solución. Además, las muestras sólidas se analizan directamente mediante vaporización electrotérmica o láser y ablación por chispa.
La espectrometría de emisión de plasma puede identificar y cuantificar cualitativamente unos 60 elementos de la tabla periódica.
Sin embargo, elementos como el boro, el carbono, el nitrógeno, el fósforo y el azufre requieren un espectrómetro de vacío porque sus líneas de emisión caen por debajo de los 180 nm, lo que introduce interferencias de los componentes atmosféricos.
La detección de metales alcalinos plantea desafíos debido a las condiciones de funcionamiento inadecuadas y sus líneas de emisión de infrarrojo cercano.
En espectroscopia de emisión de plasma, las curvas de calibración son gráficos lineales de señales eléctricas proporcionales a la intensidad de la línea frente a la concentración de analito.
Para el análisis cuantitativo, se suelen utilizar patrones externos o internos, con líneas de emisión similares e interferencias químicas con el analito.
Sin embargo, se prefieren los estándares internos cuando factores como la temperatura de la fuente de excitación y la eficiencia de la atomización afectan la intensidad de la emisión más allá de su control.

espectroscopia de emisión atómica: laboratorio

La Espectroscopia de emisión atómica (AES) es una técnica analítica potente, especialmente eficaz cuando se utiliza con fuentes de plasma, que produce espectros abundantes en líneas de emisión características. El plasma acoplado inductivamente (ICP), en particular, produce datos analíticos cuantitativos superiores debido a su alta estabilidad, bajo ruido, bajo fondo e interferencias mínimas en condiciones experimentales óptimas. Sin embargo, las fuentes de microondas operadas por aire más nuevas están surgiendo como alternativas prometedoras que podrían ser más rentables que las fuentes ICP convencionales. La AES se utiliza principalmente para analizar muestras líquidas. Sin embargo, la emisión de plasma también permite el análisis directo de muestras sólidas, lo que se puede lograr a través de varios procedimientos como la vaporización electrotérmica, la ablación por láser y chispa y la vaporización por descarga luminiscente.
En teoría, todos los elementos metálicos se pueden determinar mediante espectrometría de emisión de plasma. La eficacia de este método para los metales alcalinos es limitada debido a las difíciles condiciones de funcionamiento y la ubicación de sus prominentes líneas espectrales en la región del infrarrojo cercano. Esto puede provocar problemas de detección en muchos espectrómetros de plasma diseñados principalmente para la radiación ultravioleta. Como resultado, la espectroscopia de emisión de plasma generalmente se limita a determinar aproximadamente 60 elementos. La mayoría de los elementos tienen varias líneas prominentes adecuadas para la identificación y cuantificación. Por lo tanto, normalmente es posible identificar una línea adecuada para determinar cualquier elemento. La elección de la línea depende de la evaluación de qué otros elementos podrían estar presentes en la muestra. La posible superposición de las líneas de otros elementos debe evitarse durante la selección de la línea para el elemento de interés.
Por lo general, se puede encontrar una línea adecuada para cada elemento, y la selección se basa en la superposición con líneas de otros elementos presentes en la muestra.
Las fuentes de plasma a menudo producen curvas de calibración lineales, pero pueden producirse desviaciones de la linealidad debido a factores como la autoabsorción, correcciones de fondo erróneas, ionización y respuestas no lineales de los sistemas de detección. Cuando sea posible, los análisis cuantitativos se realizan mejor utilizando estándares externos. Sin embargo, muchos parámetros pueden afectar significativamente la intensidad de la emisión, incluida la temperatura de la fuente de excitación y la eficiencia de atomización. En los casos en que las variaciones en los parámetros de la fuente son difíciles de controlar, se pueden utilizar estándares internos.

Espectroscopia de emisión atómica: laboratorio

AES es una poderosa técnica analítica, especialmente efectiva cuando se usa con fuentes de plasma, produciendo abundantes espectros en líneas de emisión características. ...

Education

Educación

JoVE Core

Analytical Chemistry

Química Analítica

Unit 1

Unidad 1

Atomic Spectroscopy

Espectroscopía Atómica

KEY TERMS AND CONCEPTS

TÉRMINOS Y CONCEPTOS CLAVE

plasma sources in atomic emission spectroscopy

Atomic emission spectroscopy uses plasma sources to identify elements by their light. The method creates abundant spectra with characteristic emission lines. Inductively coupled plasma, or ICP, is especially useful because it gives strong quantitative data. It works well under stable conditions, with low noise, low background, and few interferences.

Newer air-operated microwave sources are also being studied. These sources may be more cost-effective than conventional ICP systems. Atomic emission spectroscopy is mainly used for liquid samples, but plasma emission can also be used for solid samples. Direct solid analysis can be done with electrothermal vaporization, laser and spark ablation, or glow-discharge vaporization.

In theory, plasma emission spectrometry can determine all metallic elements. In practice, its use for alkali metals is limited. Their strong spectral lines are in the near-infrared region, which can be hard to detect in many instruments made mainly for ultraviolet light. Because of this, plasma emission spectroscopy is usually used for about 60 elements.

Most elements have several strong lines that can help with both identification and measurement. A good line is usually chosen by checking overlap with lines from other elements in the sample. Plasma sources often give linear calibration curves, but some curves can deviate from linearity. This can happen because of self-absorption, background correction errors, ionization, or detector response problems.

When possible, quantitative analysis is best done with external standards. These are known samples used to compare emission intensity. Emission intensity can also change with source temperature and atomization efficiency, which is how well a sample turns into atoms. When source conditions are hard to keep constant, internal standards can help improve the measurement.

Plasma Sources in Atomic Emission Spectroscopy

plasma-sources-in-atomic-emission-spectroscopy

atomic spectroscopy: absorption, emission, and fluorescence

atomic-spectroscopy-absorption-emission-and-fluorescence

Element Analysis with Atomic Light Spectra

Atomic spectroscopy is used to identify and measure elements by their light signals. It supports both qualitative analysis, which tells what is present, and quantitative analysis, which tells how much is present. The optical methods in this group are atomic absorption spectroscopy, atomic emission spectroscopy, and atomic fluorescence spectroscopy. These methods begin with atomization. In this step, a solid, liquid, or solution sample is changed into gas-phase atoms and ions. Once the sample...

atomic spectroscopy: effects of temperature

atomic-spectroscopy-effects-of-temperature

How Heat Changes Atomic Spectra

Heat changes atomic spectra by affecting atomization, excitation, ionization, and line shape. In atomic spectroscopy, atomization means converting a sample into gas-phase atoms and ions. The flame temperature needed for this step changes how efficiently the method works and shifts the balance between excited and ground-state atoms. At thermal equilibrium, the relative numbers of excited and ground-state atoms can be estimated with the Maxwell-Boltzmann distribution. A small temperature rise...

atomic absorption spectroscopy: overview

Atomic Absorption Spectroscopy for Trace Metals

Atomic absorption spectroscopy (AAS) measures trace metals by tracking how atoms absorb electromagnetic radiation (EMR). The absorbed light makes atoms move to a higher-energy orbit. In AAS, the key step is atomization, which converts the sample into gas-phase atoms. A flame or a furnace is typically used for this step. Inside the flame, some atoms become thermally excited. Most stay in the ground state, which is the lowest-energy state. These ground-state gas-phase atoms absorb EMR only when...

atomic absorption spectroscopy: instrumentation

atomic-absorption-spectroscopy-instrumentation

Atomic Absorption Spectroscopy: Key Parts

Atomic absorption spectrophotometry uses a set of key parts to measure light absorbed by atoms. The main components are a radiation source, an atomizer, a monochromator, and a detector. Each part has a specific job in the instrument. The radiation source is usually a hollow-cathode lamp, or HCL, or an electrodeless-discharge lamp, or EDL. Both produce a narrow emission line at the needed wavelength. Some instruments instead use continuum sources with high-resolution monochromators to narrow...

atomic absorption spectroscopy: radiation and light sources

atomic-absorption-spectroscopy-radiation-and-light-sources

Choosing Light Sources for AAS Measurements

Atomic absorption spectroscopy (AAS) uses a light source that emits a very narrow range of wavelengths. This is needed because the source must match the absorption behavior of the analyte atom. The choice of source also depends on giving a precise, intense emission at the right wavelength for accurate detection. Two common line sources in AAS are hollow-cathode lamps (HCLs) and electrodeless-discharge lamps (EDLs). These lamps are selected based on the element being analyzed and the level of...

atomic absorption spectroscopy: atomization methods

atomic-absorption-spectroscopy-atomization-methods

Atomic Absorption Spectroscopy: Sample Atomization

Atomic absorption spectroscopy uses sample atomization to turn a liquid specimen into free atoms for analysis. The two main approaches are flame atomization and electrothermal atomization. Both methods prepare the sample so its atoms can be measured by the instrument. Flame atomization uses a nebulizer and spray chamber to mix the sample with a fuel-oxidant mixture. This creates a fine aerosol mist that enters the burner. The fuel and oxidant are usually close to a stoichiometric ratio, but a...

atomic absorption spectroscopy: interference

atomic-absorption-spectroscopy-interference

Correcting Errors in Atomic Absorption Spectroscopy

Atomic absorption spectroscopy interference can create systematic error by making the analytical signal or the background look stronger or weaker than it should be. In atomic absorption measurements, these errors are usually grouped as spectral interference, chemical interference, and physical interference. Spectral interference happens when signals from other elements or molecules overlap with the analyte signal, the substance being measured. This overlap can falsely raise or hide the analyte...

atomic absorption spectroscopy: lab

AAS Sample Prep and Calibration

Atomic absorption spectroscopy (AAS) depends on careful sample preparation and calibration. Samples must be turned into clear solutions before measurement. That often means extra treatment to dissolve materials such as soils, animal tissues, and minerals. Common sample prep methods include hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, and fusion with reagents. These steps help make the sample suitable for AAS analysis. Solutions that contain organic...

atomic emission spectroscopy: overview

Atomic Emission Spectroscopy for Element Analysis

Atomic emission spectroscopy is used to identify the elements in a sample by measuring the light they emit. Atoms are first excited to higher energy levels by thermal energy from hot sources such as plasma, arcs, or sparks. When they fall back to lower energy levels, they give off light at specific wavelengths. Each element produces its own set of lines, so the emission spectrum can show both what is present and how much is there. AES instruments are similar to atomic absorption spectrometers,...

atomic emission spectroscopy: instrumentation

atomic-emission-spectroscopy-instrumentation

AES Sample Handling and Detection

Atomic emission spectrometry (AES) uses sample handling, atomization, and detection parts to measure emitted light from atoms and ions. The sample must first be changed into a gas-phase form so it can produce a measurable signal. AES uses two main atomizer types. Continuous atomizers, such as flames and plasmas, feed the sample in a steady stream. Discrete atomizers add one sample at a time with syringes or autosamplers. The electrothermal atomizer is the most common discrete type. Many...

atomic emission spectroscopy: interference

atomic-emission-spectroscopy-interference

Reducing Interference in AES Signals

Atomic emission spectroscopy (AES) can produce interfering signals when atoms and molecules in a flame or plasma emit overlapping light. High-temperature atomizers excite many species at once, including oxides, hydroxides, and flame combustion products. These extra emissions can make the analyte signal harder to read. Spectral interference happens when emission lines or bands overlap. One way to reduce it is to improve instrument resolution. Another is to choose a different emission line for...

inductively coupled plasma atomic emission spectroscopy: principle

inductively-coupled-plasma-atomic-emission-spectroscopy-principle

ICP-AES Torch and Sample Analysis

Inductively coupled plasma atomic emission spectroscopy uses a plasma torch to analyze elements in a sample. This method is also called inductively coupled plasma optical emission spectroscopy, or ICP-OES. It is widely used because the plasma creates a strong environment for elemental analysis. The ICP source, or torch, contains three concentric quartz tubes. Argon gas flows through the tubes. A spark from a Tesla coil starts the ionization of argon and creates a high-temperature plasma. The...

inductively coupled plasma atomic emission spectroscopy: instrumentation

inductively-coupled-plasma-atomic-emission-spectroscopy

ICP-AES Instrument Types and Detection

Inductively coupled plasma atomic emission spectroscopy, or ICP-AES, uses an inductively coupled plasma to detect and analyze elements in a sample. The method measures the light given off by atoms, which helps identify and compare different elements. It is a common approach in atomic emission spectroscopy. ICP-AES instruments are built in three main forms: sequential, simultaneous multichannel, and Fourier transform instruments. Fourier transform designs are less commonly used, but they are...

atomic emission spectroscopy: lab

Atomic emission spectroscopy uses plasma sources to identify elements by their light. The method creates abundant spectra with characteristic emission lines. Inductively coupled plasma, or ICP, is especially useful because it gives strong quantitative data. It works well under stable conditions, with low noise, low background, and few interferences. Newer air-operated microwave sources are also being studied. These sources may be more cost-effective than conventional ICP systems. Atomic...

atomic fluorescence spectroscopy

Measuring Elements by Atomic Fluorescence

Atomic fluorescence spectroscopy measures elements by detecting the light atoms give off after they are excited by electromagnetic radiation. The atoms are first placed in a flame, furnace, or plasma. They absorb energy, become excited, and then release that energy as they return to their original state. The emitted light is called fluorescence. The fluorescence is observed at a right angle to the incoming beam. This setup helps separate the emitted light from the light used to excite the...

flame photometry: overview

Flame Emission Spectrometry Basics

Flame emission spectrometry uses a flame to excite elements in a sample so their light can be measured. It is also called flame photometry. The method can be used for both qualitative and quantitative analysis of elements. The idea became established in the early 1860s when Kirchhoff and Bunsen showed that specific elements give off characteristic radiation in a flame. The first instrument for this purpose measured sodium in plant ash with a Bunsen flame. A major problem was how to add the...

flame photometry: lab

Flame Photometry and Metal Detection

Flame photometry is a method for detecting metals by measuring the light they emit in a flame. A solution such as potassium chloride is aspirated into the flame. The solvent evaporates first. The remaining salt becomes dehydrated and then dissociates into free gaseous atoms in the ground state. Some of these atoms absorb energy from the flame and move to an excited state. When they return to the ground state, they emit photons at characteristic wavelengths. These emissions are very narrow...

key terms and concepts

Learn how plasma sources improve atomic emission spectroscopy, from ICP analysis to solid sample methods, calibration, and internal standards.

Plasma Sources in Atomic Emission Spectroscopy | JoVE K12

analytical chemistry

JoVE Core Analytical Chemistry simplifies complex chemical principles by breaking them down into clear, engaging animations, making topics from SI Units to NMR spectroscopy more accessible and straightforward. Additionally, the scientist-in-action videos highlight the application of these concepts in real-world research conducted in laboratories globally.

Louisiana Student Standards for Science

High School

Earth Science

Earth’s Place In The Universe

HS-ESS1-2

Chemistry

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Motion And Stability: Forces And Interactions

HS-PS2-6

Texas Essential Knowledge and Skills

Physics

PHYS.8

PHYS.8.F

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South Carolina College and Career Ready Science Standards

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Waves and Their Applications in Technologies for Information Transfer

C-PS4-4

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The Principle of Superposition and the Gravitational Field

The principle of superposition applies to gravitational forces of objects that are sufficiently far apart. It states that the net gravitational force on a ...

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Escape Velocity

The escape velocity of an object is defined as the minimum initial velocity that it requires to escape the surface of another object to which it is ...

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Circular Orbits and Critical Velocity for Satellites

The Moon orbits around the Earth. In turn, the Earth (and other planets) orbit the Sun. The space directly above our atmosphere is filled with artificial ...

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Energy of a Satellite in a Circular Orbit

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Kepler's First Law of Planetary Motion

In the early 17th century, German astronomer and mathematician Johannes Kepler postulated three laws for the motion of planets in the solar system. He ...

Kepler's Second Law of Planetary Motion

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Kepler's Third Law of Planetary Motion

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Nuclear Fusion

The process of converting very light nuclei into heavier nuclei is also accompanied by the conversion of mass into large amounts of energy, a process ...

Nuclear Power

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Nuclear Fission

Many heavier elements with smaller binding energies per nucleon can decompose into more stable elements that have intermediate mass numbers and larger ...

Nuclear Transmutation

Nuclear transmutation is the conversion of one nuclide into another. It can occur by the radioactive decay of a nucleus, or the reaction of a nucleus with ...

Atomic Structure

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Radioactivity and Nuclear Equations

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Nuclear Stability

Protons and neutrons, collectively called nucleons, are packed together tightly in a nucleus. With a radius of about 10&#8722;15 meters, a nucleus is ...

Students who demonstrate understanding can develop a model based on evidence to illustrate the life span of the sun and the role of nuclear fusion in the sun's core to release energy in the form of radiation.  Clarification Statement: Emphasis is on the energy transfer mechanisms that allow energy from nuclear fusion in the sun's core to reach Earth.  Examples of evidence for the model include observations of the masses and lifetimes of other stars, as well as the ways that the sun's radiation varies due to sudden solar flares ("space weather"), the 11-year sunspot cycle, and non-cyclic variations over centuries.  Assessment Boundary: Assessment does not include details of the atomic and sub-atomic processes involved with the sun's nuclear fusion.

3.3.9-12.A

Emission Spectra

When solids, liquids, or condensed gases are heated sufficiently, they radiate some of the excess energy as light. Photons produced in this manner have a ...

Atomic Spectroscopy: Absorption, Emission, and Fluorescence

Atomic spectroscopy is a vital tool in elemental analysis, both qualitatively and quantitatively. It can be broadly divided into optical spectroscopy, ...

Detection of Black Holes

Although black holes were theoretically postulated in the 1920s, they remained outside the domain of observational astronomy until the 1970s.
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Space-Time Curvature and the General Theory of Relativity

In 1905, Albert Einstein published his special theory of relativity. According to this theory, no matter in the universe can attain a speed greater than ...

Schwarzschild Radius and Event Horizon

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The Wave Nature of Light

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Nuclear Binding Energy

The difference between the calculated and experimentally measured masses is known as the mass defect of the atom. In the case of helium-4, the mass defect ...

Metal Flame Emission

Metal Flame Emission
Flame testing is an analytical technique where a sample is placed in a flame, and the characteristic flame color is used to identify ...

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Molecular Spectroscopy: Absorption and Emission

Molecules possess discrete energy levels called quantum states. Unlike atoms, which have simpler energy levels, molecules possess additional rotational ...

Atomic Emission Spectroscopy: Instrumentation

The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase ...

Atomic Absorption Spectroscopy: Radiation and Light Sources

Atomic absorption spectroscopy (AAS) relies on the Beer-Lambert law, which requires that the radiation source emits a narrow range of wavelengths to match ...

Students who demonstrate understanding can construct an explanation of the Big Bang theory based on astronomical evidence of light spectra, motion of distant galaxies, and composition of matter in the universe.  Clarification Statement: Emphasis is on the astronomical evidence of the red shift of light from galaxies as an indication that the universe is currently expanding, the cosmic microwave background as the remnant radiation from the Big Bang, and the observed composition of ordinary matter of the universe, primarily found in stars and interstellar gases (from the spectra of electromagnetic radiation from stars), which matches that predicted by the Big Bang theory (3/4 hydrogen and 1/4 helium).  Assessment Boundary:

3.3.9-12.B

Spectrophotometry: Introduction

Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a ...

Atomic Emission Spectroscopy: Overview

Atomic emission spectroscopy (AES) is an analytical technique used to determine the elemental composition of a sample by analyzing the light emitted from ...

ultraviolet-and-visible-uvvis-spectroscopy-overview

Ultraviolet and Visible (UV&ndash;Vis) Spectroscopy: Overview

Ultraviolet&#8211;visible (UV&#8211;visible or UV&#8211;Vis) spectroscopy is an analytical technique that investigates the interaction between matter and ...

AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. ...

Flame Photometry: Overview

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The Greek philosopher Democritus proposed that everything on Earth is made up of tiny particles called atomos, Greek for &#34;indivisible,&#34; from which ...

Students who demonstrate understanding can communicate scientific ideas about the way stars, over their life cycle, produce elements.  Clarification Statement: Emphasis is on the way nucleosynthesis, and therefore the different elements created, varies as a function of the mass of a star and the stage of its lifetime.  Assessment Boundary: Details of the many different nucleosynthesis pathways for stars of differing masses are not assessed.

Tidal Forces

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Students who demonstrate understanding can use mathematical or computational representations to predict the motion of orbiting objects in the solar system.  Clarification Statement: Emphasis is on Newtonian gravitational laws governing orbital motions, which apply to human-made satellites as well as planets and moons.  Assessment Boundary: Mathematical representations for the gravitational attraction of bodies and Kepler's Laws of orbital motions should not deal with more than two bodies, nor involve calculus.

3.3.9-12.D

Plasma Sources in Atomic Emission Spectroscopy

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