Carotid Artery and Jugular Vein Interposition: A Technique to Induce Venous Intimal Hyperplasia in Rabbit Model

Abstract

Source: Nishio, H., et al. A Rabbit Venous Interposition Model Mimicking Revascularization Surgery using Vein Grafts to Assess Intimal Hyperplasia under Arterial Blood Pressure J. Vis. Exp. (2020).

This video demonstrates the interposition of the rabbit carotid artery with the jugular vein to induce venous intimal hyperplasia by subjecting the veins to arterial pressure. This model will help investigate molecular and genetic interventions to attenuate the progression of intimal hyperplasia after revascularization surgeries.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Interposing the carotid artery by the harvested jugular vein

1. Begin with an anesthetized rabbit. Incise 50–60 mm of the cervical region with surgical scalpel longitudinally. Bluntly dissect the subcutaneous tissues and fascia. Expose a 20–30 mm segment of the ipsilateral carotid artery. Separate the artery carefully from the vein and nerve nearby. Ligate all the branches of the exposed vein with 4-0 silk suture.
2. Administer heparin sodium intravenously (200 I....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
10% Povidone-iodine solution	Nakakita	872612	Surgical expendables
2-0 VICRYL Plus	Johnson and Johnson	VCP316H	Surgical expendables
4-0 Silk suture	Alfresa pharma	GA04SB	Surgical expendables
8-0 polypropylene suture	Ethicon	8741H	Surgical expendables
Fogarty Catheter (2Fr)	Edwards Lifesciences LLC	E-060-2F	Surgical expendables
Heparin	Nipro	873334	Anticoagulant
Intravenous catheter (20G)	Terumo	SR-OT2051C	Surgical expendables