Technique to Induce Post-Traumatic Seizures in a Mouse Model via a Weight Drop Traumatic Injury

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Weight drop procedure

1. Place the mouse in an induction chamber. Set the flow of oxygen and vacuum to 1 L/min and the level of isoflurane gas to 3%−5%. Anesthetize the mouse for 5 min.
2. Remove the mouse from the induction chamber and place it on a foam pad. Test for the absence of a response to a toe or tail pinch.
3. Administer an analgesic (0.1 mg/kg buprenorphine) subcutaneously. If the electroencephalography (EEG) surgery is performed that s....

Results

Figure 1: The mouse model of repetitive diffuse traumatic brain injury (TBI). (A) Weight drop device. (a1) Weight drop tube. (a2) A 100 g weight rod. (a3) Pin holding the rod. (a4) S.......

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Buprenorphine	Par Pharmaceuticals, Cos. Inc., Spring Valley, NY, USA	60969
C57BL/6 mice	Harlan/Envigo Laboratories Inc		male, 12-16 weeks old
C57BL/6 mice	The Jackson Laboratory		male, 12-16 weeks old
Carprofen	Zoetis Services LLC, Parsippany, NJ, USA	26357	NOTE: this drug is added during weight drop only if stereotactic electrode implantation will be performed on the same day
Fluriso liquid for inhalation anesthesia	MWI Veterinary Supply Co., USA	502017
Stainless steel disc			Custom made
Sterile poly-lined absorbent towels pads	Cardinal Health, USA	3520