Chip Loading Procedure
Chip Loading Procedure - Related Videos
06:10
Accessible Silicone Chip-to-Membrane Sealing Procedure for Flexible, Reliable Bonding
0 Views •
2026
A novel sealing protocol to produce water-tight silicone-based seals for membranes of Polydimethylsiloxane (PDMS) chip devices. Intended for labs setting-up chip models and small-scale chip platform development. We demonstrate the protocol using plastic and native tissue membranes. This procedure only requires PDMS, toluene, mold, membrane, and a vacuum chamber.
10:55
Procedure for the Development of Multi-depth Circular Cross-sectional Endothelialized Microchannels-on-a-chip
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Cited by 5 •
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A microchannels-on-a-chip platform was developed by the combination of photolithographic reflowable photoresist technique, soft lithography, and microfluidics. The endothelialized microchannels platform mimics the three-dimensional (3D) geometry of in vivo microvessels, runs under controlled continuous perfusion flow, allows for high-quality and real-time imaging and can be applied for microvascular research.
08:04
A Semiautomated ChIP-Seq Procedure for Large-scale Epigenetic Studies
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Cited by 1 •
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This paper describes a semiautomated, microscaled ChIP-Seq protocol of DNA regions associated with a specific histone modification (H3K27ac) using a ChIP liquid-handler platform, followed by library preparation using tagmentation. The procedure includes control assessment for quality and quantity purposes and can be adopted to other histone modifications or transcription factors.
07:50
Chip-Based Digital PCR to Detect Rare Transcript Variants Using a Nanofluidic Chip
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2025
This video demonstrates chip-based digital PCR — a variation of the digital PCR technique that is useful in detecting rare transcript variants. The PCR reaction is partitioned into the chambers of a nanofluidic chip, each of which acts as an independent reaction. The detection of fluorescence signals from the chambers with amplified targets confirms the presence of rare transcript variants in the sample.
11:23
Lensless Fluorescent Microscopy on a Chip
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Cited by 8 •
2011
A lensless on-chip fluorescent microscopy platform is demonstrated that can image fluorescent objects over an ultra-wide field-of-view of e.g., >0.6-8 cm2 with <4μm resolution using a compressive sampling based decoding algorithm. Such a compact and wide-field fluorescent on-chip imaging modality could be valuable for high-throughput cytometry, rare-cell research and microarray-analysis.
FAQs
Pressure and flow control determine how material advances through the chip’s internal channels and chambers. Adjusting these conditions helps regulate the movement of a measured sample volume and supports more even distribution across the intended regions. This control matters because inconsistent delivery can alter the conditions needed for downstream reactions, separations, or cell-based measurements.
Priming prepares the device before the sample or reagent enters its defined pathways. This step supports consistent movement through the channels and chambers while helping prevent air bubbles from entering the loaded regions. Maintaining those conditions is important because bubbles, leaks, or uneven filling can interfere with the intended reaction, separation, or cell-handling environment.
A measured volume helps control how much sample, reagent, or cell suspension reaches the designated regions of the device. More consistent delivery supports comparable reaction or separation conditions between experiments. In applications such as molecular assays and single-cell analysis, loading accuracy can therefore influence reproducibility and the quality of the resulting data.
Preventing cross-contamination keeps the material introduced through an inlet associated with its intended channel or chamber. This is especially important when different samples, reagents, or cells must remain distinguishable within the experiment. Maintaining separation protects the planned conditions for analysis and helps researchers interpret results without unintended material affecting another region or measurement.
A typical workflow begins by priming the chip, followed by introducing the biological sample, reagent, or cells through an inlet. The operator controls pressure or flow while dispensing a measured volume and observes the loading process for bubbles, leaks, or cross-contamination. The goal is to leave material distributed consistently in the intended channels and chambers before analysis.
The loading process should be monitored for stable movement through the defined pathways, accurate delivery of the intended volume, and consistent distribution among the relevant chambers or channels. Operators also need to watch for air bubbles, leaks, and cross-contamination. These checks help preserve the planned reaction or separation conditions before the chip proceeds to analysis.
Chip loading supports several biological techniques, including cell handling, molecular assays, droplet generation, and single-cell analysis. The appropriate material may be a biological sample, reagent, or cells, depending on the experiment. Across these applications, careful loading provides the starting conditions needed for controlled processing and can affect reproducibility and data quality.