Electroporation Cuvette
Electroporation Cuvette - Related Videos
12:19
Bacterial Transformation: Electroporation
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2023
The term “transformation” refers cellular ingestion of foreign DNA. In nature, transformation can occur in certain types of bacteria. In molecular biology, however, transformation is artificially induced through the creation of pores in the bacterial cell walls. Bacterial cells that are able to take up DNA from the environment are called competent cells. Electrocompetent cells can be produced in the laboratory and transformation of these cells can be achieve via the application of an...
11:57
Electroporation of Mycobacteria
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Cited by 55 •
2008
Mycobacterial pathogenic strategies remain poorly understood. The slow growth rate of most species, the impenetrable nature of the cell-wall, and the hazards of working with pathogens make mycobacteria difficult to study and are largely responsible for our poor understanding of these organisms. In this video we will demonstrate the technique of electroporation, which involves subjecting cells to a brief high electrical impulse to allow the entry of DNA. It is the most widely used method for...
04:55
Electroporation-Based Delivery of Fluorescent Biomolecules into Bacterial Cells
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2025
Source: Aigrain, L., et al. Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation. J. Vis. Exp. (2015)The video demonstrates the delivery of fluorescent biomolecules into electrocompetent microbial cells using electroporation. The process begins with the introduction of fluorophore-tagged DNA or proteins into a chilled cell suspension. A high-voltage pulse is then applied to create temporary membrane pores, enabling biomolecule entry into the...
04:41
Introduction of Bacterial Effector Proteins into Mammalian Cells by Electroporation
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2025
In this video, we demonstrate the introduction of bacterial effector proteins into mammalian cells by electroporation. The intracellular localization of the bacterial effector proteins is detected by confocal microscopy following staining with specific fluorophore-conjugated antibodies.
05:11
Organoid Electroporation: A Method to Transfect Plasmid DNA into Gastrointestinal Organoids
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2023
This video describes the electroporation protocol for transfecting large plasmids into three-dimensional gastrointestinal organoids to prove its universal functionality in different entities, namely pancreatic ductal adenocarcinoma (PDAC), colorectal cancer (CRC), cholangiocarcinoma (CCC), and gastric cancer (GC) organoids.
FAQs
The cuvette positions the cell suspension between two electrodes so the brief, high-voltage pulse crosses the sample. This pulse temporarily disrupts the membrane and forms pores that permit DNA, RNA, or other molecules to enter. The desired outcome depends on creating access for the cargo while preserving conditions that allow the membrane to reseal and the cells to remain viable.
Pore formation provides only a temporary entry route. After the pulse, cells need suitable recovery conditions so their membranes can reseal rather than remain disrupted. Successful resealing supports continued viability, growth, or expression of introduced material. Thus, electroporation is not limited to cargo entry; recovery determines whether treated cells can resume useful biological activity.
The same cuvette-based approach can support transformation or transfection in bacteria, yeast, and mammalian cells, but the biological outcome is interpreted differently across these systems. Introduced nucleic acids may support transformation, transfection, gene editing, recombinant protein production, or cellular research. The cell type therefore determines the experimental purpose and the outcome researchers evaluate after recovery.
A typical workflow places a cell suspension containing the selected DNA, RNA, or other cargo into the sterile cuvette, applies a brief high-voltage pulse through the electrodes, and then provides suitable recovery conditions. The sequence matters: the pulse enables temporary entry, while the recovery period gives the membrane an opportunity to reseal and the cells to resume growth or expression.
The system can deliver nucleic acids such as DNA and RNA, as well as other molecules identified as compatible cargo for electroporation. Once inside cells, these materials can support different experimental goals, including gene editing, recombinant protein production, or cellular research. The resulting readout may involve cell growth, expression, or another response associated with the introduced material.
They are useful when researchers need to introduce material into bacterial, yeast, or mammalian cells for transformation or transfection. Applications described for this approach include gene editing, recombinant protein production, and studies of cellular behavior or function. The cuvette provides a sterile setting for the pulse and sample, linking controlled delivery with downstream biological analysis.