Pbmc Culture
Pbmc Culture - Related Videos
11:40
A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMC
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Cited by 7 •
2018
We present a protocol to investigate the crosstalk between X-ray-irradiated Caco-2 and peripheral blood mononuclear cells (PBMC). The protocol starts with Caco-2 irradiation and set-up of the co-culture with PBMC; subsequently, trans-epithelial electrical resistance is measured regularly over 48 h and western blot performed in both Caco-2 and PBMC.
02:26
A Technique to Generate a Human PBMC-Engrafted Humanized Xenograft Mouse Model
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2025
This video demonstrates an assay to generate a humanized xenograft mouse model. An immunocompromised mouse is injected with cyclophosphamide to cause immune cell depletion. Patient-derived tumor cells mixed with human peripheral blood mononuclear cells (PBMCs) in a hydrogel are subcutaneously injected into the mouse, forming a tumor with infiltrating T cells.
08:39
Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro
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Cited by 27 •
2010
T lymphocyte migration occurs during homing to lymphoid organs, exit from the vasculature, and entering into peripheral tissues. Here, we describe a protocol that can be used to analyze T lymphocyte migration in vitro.
08:17
A Human Peripheral Blood Mononuclear Cell (PBMC) Engrafted Humanized Xenograft Model for Translational Immuno-oncology (I-O) Research
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Cited by 6 •
2019
We describe a human peripheral blood mononuclear cell (PBMC) — based humanized xenograft mouse model for translational immuno-oncology research. This protocol could serve as a general guideline for establishing and characterizing similar models for I-O therapy assessment.
08:11
Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.
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Cited by 1 •
2015
Unlike cell-free HIV-1 particles, infected CD4+ T cells are effectively sensed by plasmacytoid dendritic cells (pDCs). This manuscript describes a method where peripheral blood mononuclear cells (PBMCs) or isolated pDCs are co-cultured with HIV-1 infected T cells to evaluate innate sensing by pDCs as assessed by release of type-I IFN.
FAQs
Because the culture retains primarily lymphocytes and monocytes, researchers can examine responses from more than one immune-cell population in the same experimental system. This diversity makes it possible to study cell signaling and immune-cell interactions alongside individual outcomes such as proliferation, cytokine secretion, and cytotoxicity, providing a broader view of human immune-cell behavior.
The selected stimulus helps define the response under investigation. Mitogens can activate cellular responses broadly, whereas antigens, cytokines, or other stimuli provide different experimental inputs. By choosing among these conditions, researchers can focus on responses relevant to activation, signaling, cytokine production, proliferation, cytotoxicity, or interactions between immune-cell populations.
PBMC culture supports several complementary readouts rather than a single endpoint. Researchers may assess whether cells proliferate, which cytokines they secrete, how signaling changes, whether cytotoxic activity occurs, and how immune-cell populations interact. Together, these measurements help characterize both the behavior of individual cellular responses and the coordinated activity of the mixed immune-cell system.
A typical workflow begins with peripheral blood, followed by isolation of peripheral blood mononuclear cells through density-gradient centrifugation. The recovered cells are then maintained in suitable culture medium under controlled laboratory conditions. Researchers may subsequently add a mitogen, antigen, cytokine, or another stimulus when the experiment requires activation of a defined immune response.
Researchers use this ex vivo model when they need to investigate human immune-cell behavior in a controlled laboratory setting while retaining diverse immune-cell populations. Supported applications include studies of infection, inflammation, immunotherapy, vaccine responses, and drug effects. The approach can therefore connect cellular measurements with biologically relevant immune processes.
Researchers can expose cultured cells to relevant stimuli or treatment conditions and then examine outcomes such as proliferation, cytokine secretion, signaling, cytotoxicity, and immune-cell interactions. These measurements provide experimental evidence about how a treatment or immune challenge affects human immune cells, making the culture useful for comparing cellular responses across infection, vaccination, immunotherapy, or drug-related studies.