Three Laser Heterodyne Technique

The three-laser heterodyne technique is a coherent spectroscopic method that uses three laser fields to measure molecular responses with high frequency and phase sensitivity. In operation, selected laser beams interact with a sample to create a weak, frequency-shifted optical signal, which is mixed with a reference field; the resulting beat signal encodes the response’s amplitude, phase, and resonance frequencies. In chemistry, this approach supports sensitive measurements of vibrational or rotational transitions, reaction intermediates, and molecular dynamics, while heterodyne detection can improve discrimination of weak signals against background noise. It is useful for studying structure, kinetics, and light-driven chemical processes.

Three Laser Heterodyne Technique - Related Videos

Research

JoVE Journal - Engineering

Characterizing Far-infrared Laser Emissions and the Measurement of Their Frequencies

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Cited by 4 •

2015

We describe the generation of far-infrared radiation using an optically pumped molecular laser along with the measurement of their frequencies with heterodyne techniques. The experimental system and techniques are demonstrated using difluoromethane (CH2F2) as the laser medium whose results include three new laser emissions and eight measured laser frequencies.

Probe-based Confocal Laser Endomicroscopy of the Urinary Tract: The Technique

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Cited by 25 •

2013

Probe-based confocal laser endomicroscopy enables real-time microscopy of the human urinary tract during cystoscopy, providing dynamic, intravital imaging of pathological states such as bladder cancer with cellular resolution. Endomicroscopy may augment the diagnostic accuracy of standard white light endoscopy and provide intraoperative image guidance to improve surgical resection.

Automation of Mode Locking in a Nonlinear Polarization Rotation Fiber Laser through Output Polarization Measurements

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Cited by 2 •

2016

A protocol to detect and automate mode locking in a pre-adjusted nonlinear polarization rotation fiber laser is presented. The detection of a sudden change in the output polarization state when mode locking occurs is used to command the alignment of an intra-cavity polarization controller in order to find mode-locking conditions.

An “All-laser” Endothelial Transplant

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Cited by 5 •

2015

An all laser procedure is proposed in the endothelial transplant. The surgical technique is based on the use of a femtosecond laser to prepare the donor tissue. Laser welding technique is then used to secure the donor endothelium in the correct position.

Laser Microdissection: A Technique to Isolate Invading Colorectal Cancer cells from a 3D Organotypic Culture Model

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2023

The video describes the laser dissection of the 3D organotypic co-culture model with micron-level precision. In 3D organotypic models, stromal constituents such as fibroblasts are 3-dimensionally co-cultured with cancer epithelial cells. The 3D model serves as an experimental tool that enables invasion and cancer-stroma interactions to be studied in near-physiological conditions.

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