Cfu Lung Analysis
Cfu Lung Analysis - Related Videos
08:01
Non-surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry
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Cited by 51 •
2011
We illustrate non-surgical delivery of test materials into the lungs of anesthetized mice via the trachea. This method permits lung exposure to bacterial and viral pathogens, cytokines, antibodies, beads, chemicals, or dyes. We further describe harvesting and processing of lungs and lung draining lymph nodes (LDLNs) for flow cytometry.
03:48
Mouse Lung Preparation for Flow Cytometry: Generating Cell Suspension from Lung Tissue for Flow Cytometric Analysis
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2023
This video describes the preparation of mouse lung cell suspension for flow cytometric analysis to study PD-L1 expression of lung adenocarcinoma cells.
03:31
Methyl Cellulose-Based CFU Assay: A Method to Determine the Effect of Anti-cancer Compounds on Colony Formation in Leukemic Cells
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2023
This video describes the protocol for methyl cellulose-based colony formation unit or CFU assay, which helps identify and count the number of colonies formed by leukemic cells in response to the treatment with anticancer compounds.
02:38
Lipopolysaccharide-Induced Acute Lung Injury in Mice for Exosome and Immune Analysis
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2026
Source: Yuan, Z., et al. Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury. J. Vis. Exp. (2018)This video demonstrates an in vivo mouse model for inducing and analyzing acute lung injury (ALI) using intraperitoneal administration of bacterial lipopolysaccharide. The inflammatory cascade triggered by alveolar macrophages and neutrophils leads to epithelial and endothelial barrier disruption, allowing fluid and immune cells to accumulate in the alveoli. Alveolar...
07:06
A Simple Method of Mouse Lung Intubation
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Cited by 50 •
2013
This paper describes a striaghforward and efficient method of intubating mice for pulmonary function measurements or pulmonary instillation, that allows the mice to recover and be studied at later times. The procedure involves an inexpensive fiberoptic light source that directly illuminates the trachea.
FAQs
The readout depends on growth into visible colonies, so it reflects bacteria that remain viable under the assay conditions rather than total bacterial material. A single viable cell or a clump of cells may generate one colony-forming unit, meaning CFU values represent viable units and may not equal an exact count of individual bacterial cells.
Normalization makes bacterial burdens more comparable when samples differ in size or amount. Reporting CFU relative to tissue mass or respiratory-sample volume helps separate differences in infection burden from differences in how much material was analyzed. This is especially important when comparing pulmonary infection outcomes across experimental groups or time points.
A lower viable bacterial burden can indicate more effective pulmonary clearance, whereas a higher burden can indicate bacterial growth or incomplete control of infection. In immunology and infection studies, comparing CFU outcomes helps connect host immune responses with pathogen persistence and provides a quantitative endpoint for evaluating disease severity.
The workflow begins with collecting lung tissue or a respiratory sample, followed by homogenization to distribute bacteria through the material. The homogenate is serially diluted, and measured dilutions are spread on appropriate agar. After colonies become visible, investigators use the colony counts and the dilution information to report viable bacterial burden.
Researchers can compare viable bacterial burdens between treated and untreated infection groups to determine whether an antimicrobial intervention reduces pulmonary infection. Because the result provides a direct microbiological endpoint, it supports assessment of treatment-associated bacterial clearance or persistence rather than relying only on broader indicators of disease.
In vaccine experiments, lung CFU outcomes provide a way to compare bacterial burdens after infection in vaccinated and control groups. Reduced CFU values in the vaccinated condition would be consistent with improved control of pathogen growth or enhanced clearance. The assay therefore links vaccination status to a measurable pulmonary infection outcome.