Cardiac Blood Sampling
Cardiac Blood Sampling - Related Videos
04:47
Cardiac Blood Collection via Subclavian Puncture in Mice
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2025
Subclavian cardiac puncture is a simple, reliable method for collecting terminal blood in mice. It is easy to operate and ensures high-purity samples.
05:27
Drawing Blood from Rats through the Saphenous Vein and by Cardiac Puncture
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Cited by 87 •
2007
Blood draws are necessary in a large number of studies, for example to study the pharmacokinetics profile of a compound. Here, we demonstrate how to draw blood from rats using two techniques: blood draw from the saphenous vein or by cardiac puncture.
07:11
Sampling Blood from the Lateral Tail Vein of the Rat
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Cited by 82 •
2015
Blood samples are useful for assessing biomarkers of physiological states or disease in vivo. Here we describe the methodology to sample blood from the lateral tail vein in the rat. This method provides rapid samples with minimal pain and invasiveness.
03:50
CTC Isolation from a Whole Blood Sample: A Method to Obtain CTCs from Murine Colorectal Cancer Model
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2023
This video describes a detailed protocol for isolating circulating tumor cells from the whole blood sample of a euthanized colorectal tumor mouse model. These isolated CTCs can be cultured and used for intended downstream analysis.
08:43
Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery
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Cited by 9 •
2017
Endothelial progenitor cells (EPCs) are crucially involved in the neovascularization of ischemic tissues. This method describes the isolation of human EPCs from peripheral blood, as well as the identification of their migratory potential against serum samples of cardiac surgical patients.
FAQs
Comparing blood from different locations reveals differences that a single sample may miss. Variations in oxygen content, blood gases, pressure-related findings, or biochemical markers can indicate abnormal circulation, an intracardiac shunt, impaired oxygen delivery, or regional metabolic changes. The pattern across sampling sites helps relate an abnormal result to a particular part of the cardiovascular system.
An intracardiac shunt alters the expected distribution of blood within the heart and major vessels. Sampling at several locations allows clinicians to compare oxygen content or gas measurements and identify an unusual difference between sites. This comparative approach supports recognition of abnormal blood flow and contributes to evaluating congenital heart defects.
Oxygen content and blood-gas results help assess oxygen delivery and detect unusual circulation patterns, while pressure-related differences add information about conditions across sampled cardiac locations. Biochemical markers can show regional metabolic changes. Considering these measurements together gives a broader assessment of cardiovascular function than relying on one type of laboratory result alone.
During cardiac catheterization, a catheter is guided through a blood vessel toward a selected heart chamber or major vessel. Blood is then withdrawn at the intended location for analysis. Sampling sites are chosen according to the cardiovascular question being investigated, and collecting from multiple locations permits direct comparison of circulation-related findings.
The technique is used when clinicians need detailed information about circulation, oxygen delivery, or cardiovascular function. It can support evaluation of congenital heart defects and pulmonary vascular disease, particularly when comparisons among cardiac locations may clarify abnormal flow. Results can also help assess how a patient responds to treatment.
In cardiovascular research, samples from defined heart or vessel locations provide material for examining oxygen content, gases, pressure-related differences, and biochemical markers. Comparing regional results can reveal metabolic or circulatory changes that are not apparent from a general blood sample. The findings support investigation of cardiovascular function, disease mechanisms, and treatment responses.