Peak Oxygen Uptake

Peak oxygen uptake, often reported as VO₂peak, is the highest rate at which the body consumes oxygen during strenuous exercise and a widely used measure of cardiorespiratory fitness. It is assessed during a graded exercise test, typically on a treadmill or cycle ergometer, while breath-by-breath gas analysis quantifies oxygen intake and carbon dioxide output; the value reflects integrated function of the lungs, heart, circulation, blood, and working muscles. In medicine, VO₂peak helps evaluate exercise capacity, characterize functional limitation, guide rehabilitation, and monitor responses to treatment in conditions such as cardiovascular or pulmonary disease, while also informing risk assessment and individualized exercise planning.

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Research

JoVE EoE - Assay Techniques

Radiolabeled Amino Acid Uptake Assay to Quantify Cellular Uptake of Amino Acids

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2025

This video demonstrates an in vitro technique for estimating amino acid uptake by bone marrow-derived stromal cells. Na+-dependent amino acid transporters mediate the uptake of radiolabeled amino acids inside the cells, and the cellular uptake is estimated by measuring the radioactivity.

Education

JoVE Core - Analytical Chemistry

IR Spectrum Peak Intensity: Dipole Moment

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2024

The dipole moment of a bond is the product of the partial charge on either atom and the distance between them. Dipole moments influence the efficiency of IR absorption and the peak intensity. When a bond with a dipole moment is placed in an electric field, the direction of the field determines if the bond is compressed or stretched. Electromagnetic radiation consists of an electric field component that rapidly reverses direction. It follows that polar bonds are alternately stretched and...

IR Spectrum Peak Intensity: Amount of IR-Active Bonds

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2024

When infrared radiation is passed through a molecule, absorption occurs if the molecule's vibration leads to a substantial change in its bond dipole moment. Transitions between vibrational energy levels, typically corresponding to infrared frequencies (4000–400 cm−1), allow absorption if the vibration significantly alters the dipole moment, making the molecule infrared active. The molecular bonds have different stretching and bending vibrations, resulting in various peaks with varying...

IR Spectrum Peak Broadening: Hydrogen Bonding

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2024

The vibrational frequency of a bond is directly proportional to its bond strength. As a result, stronger bonds vibrate at higher frequencies, while weaker bonds vibrate at lower frequencies. The stretching vibration of the strong O–H bond in alcohols and phenols (very dilute solution or gas phase) appears as a sharp peak at 3600–3650 cm−1. However, the extent of hydrogen bonding influences the observed stretching frequency and band broadening. Intermolecular or intramolecular hydrogen bonding...

Mitochondrial Calcium Uptake Assay: A Plate Reader-Based Method for Measuring Calcium Uptake in Isolated Mitochondria

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2025

This video describes a plate reader-based assay to measure mitochondrial calcium uptake in isolated mitochondria. The assay analyzes the fluorescence signal of a calcium-sensitive fluorescent dye to study the kinetics of mitochondrial calcium uptake and calcium overload.

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