Membrane Insert Culture
Membrane Insert Culture - Related Videos
12:58
Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons
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Cited by 8 •
2012
By tagging the extracellular domains of membrane receptors with superecliptic pHluorin, and by imaging these fusion receptors in cultured mouse neurons, we can directly visualize individual vesicular insertion events of the receptors to the plasma membrane. This technique will be instrumental in elucidating the molecular mechanisms governing receptor insertion to the plasma membrane.
07:06
Isolation and Quantification of Axonal mRNAs Using Porous Membrane Inserts and RTddPCR
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Cited by 1 •
2026
This study presents a robust method to isolate axonal mRNAs using porous membrane inserts, enabling total neuron vs. neurite separation and RNA purification. Combined with RTddPCR, the approach allows absolute quantification of low-copy transcripts, facilitating studies of mRNA transport and local translation with high sensitivity, reproducibility, and broad experimental applicability.
03:28
A Permeable Membrane Insert-Based Infection System to Study the Impact of Bacterial Toxins
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2025
This video demonstrates a permeable membrane insert-based infection system to assess the impact of secreted bacterial toxins. By eliminating direct contact between the bacterial pathogen and the host cells using a permeable membrane, this technique allows for studying the effect of toxins secreted by pathogenic bacteria on mammalian host cells.
01:12
Porin Insertion in the Outer Mitochondrial Membrane
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2023
Porins are beta-barrel proteins translocated to the mitochondrial outer membrane through the TOM complex into the intermembrane space. Porin precursors bind TIM chaperones within the intermembrane space and are guided to the Sorting and Assembly Machinery complex or SAM complex on the outer mitochondrial membrane. Three models describe the assembly of porins by the SAM complex and their insertion into the outer membrane. Model 1 suggests that porins are assembled outside the SAM channel as the...
11:56
Fabrication and Operation of an Oxygen Insert for Adherent Cellular Cultures
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Cited by 1 •
2010
Fabrication and validation of an add-on platform that offers enhanced control over the spatial and temporal oxygenation in a 6-well plate. The device is adaptable to a number of culture systems and can be used to investigate the effects of oxygen on wound healing.
FAQs
The porous membrane separates interacting populations while preserving shared access to nutrients and diffusible signaling molecules. If cells remain on opposite sides, responses can be attributed to factors released into the medium rather than physical contact. When the pores permit cellular processes to extend between compartments, researchers can also examine communication that depends on limited structural interaction.
Pore properties determine what can move between the two compartments, including nutrients, soluble molecules, and, when appropriate, cellular processes. The apical and basolateral arrangement preserves spatial organization that is lost when populations are fully mixed. This configuration is particularly useful for examining how cells respond differently according to which side of a barrier or culture environment they occupy.
A mixed culture allows interacting populations to share the same space, making physical contact and released factors difficult to separate experimentally. Membrane Insert Culture maintains distinct populations while permitting controlled exchange across the membrane. That separation provides a more physiologically organized setting for testing whether an observed neural response depends on contact, diffusion, or both.
Researchers place cells on a porous membrane insert and maintain the interacting populations in separate compartments. They then control which populations occupy the membrane or the opposing chamber, while allowing the membrane to support exchange across the system. Experimental observations can focus on communication, barrier behavior, or treatment responses without fully mixing the cultures.
This approach is useful when investigators need to study neuron-glia signaling, neural barrier behavior, or communication associated with disease-related conditions. It also supports treatment-response studies in which one cell population may influence another through shared soluble factors. Preserving separate compartments helps connect measured cellular responses to a defined interaction environment rather than an undifferentiated mixed culture.
Researchers can assess how one cell population affects another, whether communication occurs through released factors, and how cells behave in relation to a neural barrier arrangement. The system can also reveal responses to treatments or disease-related interactions while retaining compartment information. These outcomes help interpret cellular communication in a setting that is more organized than conventional mixed culture.