Photochemical Thrombosis
Photochemical Thrombosis - Related Videos
10:29
Photochemical Initiation Of Radical Polymerization Reactions
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2023
Source: David C. Powers, Tamara M. Powers, Texas A&M In this video, we will carry out the photochemically initiated polymerization of styrene to generate polystyrene, which is an important commodity plastic. We will learn the fundamentals of photochemistry and use simple photochemistry to initiate radical polymerization reactions. Specifically, in this module we will examine the photochemistry of benzoyl peroxide and its role as a photo-initiator of styrene polymerization reactions. In the...
14:54
Rat Model of Photochemically-Induced Posterior Ischemic Optic Neuropathy
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Cited by 2 •
2015
The goal of this protocol is to photochemically induce ischemic injury to the posterior optic nerve in rat. This model is critical to studies of the pathophysiology of posterior ischemic optic neuropathy, and therapeutic approaches for this and other optic neuropathies, as well as of other CNS ischemic diseases.
10:37
Ferric Chloride-induced Murine Thrombosis Models
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Cited by 56 •
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We report a refined procedure of the ferric chloride (FeCl3)-induced thrombosis models on carotid and mesenteric artery as well as vein, characterized efficiently using intravital microscopy to monitor time to occlusive thrombi formation.
05:41
Photochemical Oxidative Growth of Iridium Oxide Nanoparticles on CdSe@CdS Nanorods
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Cited by 4 •
2016
A protocol for the photochemical oxidative growth of small crystalline iridium oxide nanoparticles on the surface of CdSe@CdS seeded rod nanoparticles is presented.
10:26
A Multicenter MRI Protocol for the Evaluation and Quantification of Deep Vein Thrombosis
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Cited by 5 •
2015
The goal of this study is to use magnetic resonance venography with long-circulating gadolinium-based contrast agent and direct thrombus imaging for quantitative evaluation of DVT volume in a multicenter, clinical trial setting. Inter- and intra-observer variability assessments were conducted, and reproducibility of the protocol was determined.
FAQs
Light activation of the circulating photosensitizer generates reactive oxygen species at the illuminated vessel. These molecules damage the vascular endothelium, the cell layer lining the vessel, and the injury promotes platelet aggregation. The combined endothelial disruption and platelet response produce the localized thrombus needed to model vascular obstruction and its consequences.
Focused illumination restricts photosensitizer activation to a selected vessel or region. Because reactive oxygen species are generated where the light is directed, researchers can produce a localized vascular insult rather than broadly affecting the circulation. This spatial control supports reproducible studies of how a defined vascular disruption alters nearby brain tissue and function.
The photosensitizer provides the light-responsive component, while illumination supplies the trigger that activates it within the circulation. Rose bengal is commonly used for this purpose. Neither component is described as acting alone in the experimental sequence: their combination produces reactive oxygen species at the targeted site, initiating endothelial damage and thrombus formation.
The procedure begins by introducing a photosensitizing compound into the circulation. Researchers then direct focused light toward a selected vessel or brain region, activating the compound there. Reactive oxygen species damage the endothelium, platelet aggregation follows, and a localized thrombus develops. This sequence creates the vascular injury required for subsequent analysis.
Its spatially controlled clot formation can produce reproducible focal cerebral ischemia in animal models. This gives neuroscience researchers a defined setting for examining stroke-related neuronal damage and the effects of vascular disruption. The resulting model also supports investigation of inflammation, recovery, and potential therapies under a localized ischemic condition.
Studies can assess how localized vascular injury affects neuronal tissue, inflammatory responses, recovery, and brain structure or function. The method is also useful for testing potential therapies in the context of stroke-related ischemia. Because the insult is spatially controlled, researchers can relate observed changes to the targeted vascular disruption and its surrounding neural consequences.