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Infödda kromatin Immunoprecipitation använder murina hjärnan tumören Neurospheres

Article DOI: 10.3791/57016-v • 09:31 min • January 29th, 2018
January 29th, 2018 •
1, 1,2, 3,4, 1,2, 1,2
1Department of Cell and Developmental Biology, University of Michigan Medical School, 2Department of Neurosurgery, University of Michigan Medical School, 3Cancer Research Summer Internship Program (CARSIP), Cancer Biology Program, University of Michigan Medical School, 4Department of Biology, University of Puerto Rico-Río Piedras Campus

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Epigenetiska mekanismer är ofta förändrade i gliom. Kromatin immunoprecipitation kunde användas för att studera konsekvenserna av genetiska förändringar i gliom som uppstår genom ändringar i Histon ändringar som reglerar kromatin struktur och gen transkription. Det här protokollet beskriver infödda kromatin immunoprecipitation på murina hjärnan tumören neurospheres.

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Cancerforskning fråga 131 kromatin immunoprecipitation epigenetik gliom neurosphere generation Histon ändringar
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