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Modeling osteosarkom ved hjælp af Li-Fraumeni syndrom Patient-afledte inducerede pluripotente stamceller-celler

Article DOI: 10.3791/57664-v • 08:52 min • June 13th, 2018
June 13th, 2018 •
1,2, 1, 1,3, 1, 4, 1, 1,2,5,6
1Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, 2Graduate School of Biomedical Sciences, The University of Texas MD Anderson Cancer Center UTHealth, 3Department of Musculoskeletal Oncology, The First Affiliated Hospital of Sun Yat-sen University, 4Women's Health Institute, Cleveland Clinic Foundation, 5Center for Stem Cell and Regenerative Medicine, The Brown Foundation Institute of Molecular Medicine for the Prevention of Human Diseases, The University of Texas Health Science Center at Houston, 6Center for Precision Health, School of Biomedical Informatics and School of Public Health, The University of Texas Health Science Center at Houston

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Her præsenterer vi en protokol for generation af induceret pluripotente stamceller (iPSCs) fra Li-Fraumeni syndrom (LFS) patient afledte fibroblaster, differentiering af iPSCs via mesenchymale stamceller (msc) til osteoblaster og modellering in vivo tumordannelse med LFS patient-afledte osteoblaster.

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Kræftforskning sag 136 iPSCs Li-Fraumeni syndrom omprogrammering differentiering mesenkymale stamceller osteoblaster osteosarkom xenograft
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