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Journal / Biochemistry / Måling af nukleotidbinding til intakte,…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
Måling af nukleotidbinding til intakte, funktionelle membranproteiner i realtid
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Måling af nukleotidbinding til intakte, funktionelle membranproteiner i realtid

Article DOI: 10.3791/61401-v • 08:33 min • March 11th, 2021
March 11th, 2021 •
1, 1, 1,2
1Department of Physiology, Anatomy and Genetics, University of Oxford, 2Department of Chemistry and Neuroscience Program, Trinity College

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Denne protokol præsenterer en metode til måling af adeninnukleotidbinding til receptorer i realtid i et cellulært miljø. Binding måles som Förster-resonansenergioverførsel (FRET) mellem trinitrophenylnukleotidderivater og protein mærket med en ikke-kanonisk, fluorescerende aminosyre.

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Denne måned i JoVE udgave 169 ligandbinding membranprotein ionkanal nukleotid metode patchklemme fluorescens spektre receptor
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