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Journal / Genetics / In Vivo CRISPR/Cas9 Screening för att samtidigt…
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Genetics

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In Vivo CRISPR/Cas9 Screening för att samtidigt utvärdera genfunktionen i mushud och munhåla
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In Vivo CRISPR/Cas9 Screening för att samtidigt utvärdera genfunktionen i mushud och munhåla

Article DOI: 10.3791/61693-v • 07:52 min • November 2nd, 2020
November 2nd, 2020 •
1, 1,2, 1,2, 3, 1,2
1Centre for Molecular and Systems Biology, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, 2Department of Molecular Genetics, University of Toronto, 3Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University

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Här beskriver vi en snabb och direkt in vivo CRISPR/Cas9 screening metodik med ultraljud-guidad in utero embryonala lentiviral injektioner för att samtidigt bedöma funktioner hos flera gener i huden och munhålan hos immunkompetenta möss.

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Denna månad i JoVE utgåva 165 in vivo CRISPR ultraljud guidad mikroinjektion långsvans CRISPR-bibliotek musmodeller av cancer snabb in vivo-skärm
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