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MS2-affinitetsrensing kombinert med RNA-sekvensering i Gram-positive bakterier
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MS2-affinitetsrensing kombinert med RNA-sekvensering i Gram-positive bakterier

Article DOI: 10.3791/61731-v • 08:34 min • February 23rd, 2021
February 23rd, 2021 •
1, 2, 2, 1, 1, 1
1Université de Strasbourg, CNRS, 2Department of Biochemistry, Université de Sherbrooke

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MAPS-teknologi er utviklet for å undersøke målomet til en bestemt regulatorisk RNA in vivo. SRNA av interesse er merket med en MS2-aptamer som muliggjør samtidig rensing av sine RNA-partnere og deres identifikasjon ved RNA-sekvensering. Denne modifiserte protokollen er spesielt egnet for Gram-positive bakterier.

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Tilbaketrekning utgave 168 MAPS regulatorisk RNA RNA-sekvensering targetome regulatorisk nettverk korensing MS2-aptamer Gram-positive bakterier Staphylococcus aureus
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